| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-04-15 |
| タイトル |
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|
タイトル |
Mirror-Image D-Core Streptavidin Exhibits Reduced Immunogenicity and Comparable Biodistribution to Natural Core Streptavidin in Mice. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Masatoshi Suganuma
Aya Sugyo
Hitomi Sudo
Atsushi Tsuji
Youichi Nishikawa
|
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
The clinical application of natural core streptavidin remains unrealized owing to its immunogenicity and interference from endogenous D-biotin. These limitations could be addressed using D-core streptavidin, the mirror-image form. Here, we evaluated the immunogenicity and biodistribution of D-core streptavidin in mice. Intraperitoneal administration of natural core streptavidin induced antibodies against streptavidin, whereas no antibody production was observed for D-core streptavidin. The protease assay indicated that proteolysis by cathepsin B differed between D-core streptavidin and the natural core form. The difference in degradation by cathepsin B in non-denatured conditions may contribute to the reduced immunogenicity of D-core streptavidin. Biodistribution studies revealed no significant differences between D-core and natural core streptavidins in tumor-bearing mice. These findings support the further development of D-core streptavidin in combination with L-biotin for in vivo applications. |
| 書誌情報 |
Chemical & pharmaceutical bulletin
巻 74,
号 2,
p. 175-180,
発行日 2027-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0009-2363 |
| PubMed番号 |
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
41708102 |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1248/cpb.c25-00534 |
