| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-02-27 |
| タイトル |
|
|
タイトル |
Deep-brain thermo-endomicroscopy |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Takuma Sugi
Ryuki Imamura
Yurina Nakane
Naoya Kataoka
Abe Hiroshi
Ohshima Takeshi
Shingo Sotoma
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Subtle deviations in biophysical parameters, particularly temperature, within deep-brain regions exert substantial effects on whole-body homeostasis and metabolism. However, the underlying mechanisms remain poorly understood because cellular-resolution measurements of these parameters in the deep brain have been technically inaccessible. Here, we present a lensless fiber-optic quantum-sensing endomicroscopy that enables cellular-resolution temperature mapping in the mouse deep brain. A microwire-coupled, lensless fiber bundle permits simultaneous temperature readout from optically detected magnetic resonance spectra of >50 fluorescent nanodiamonds, achieving cellular resolution (4.88 μm) with a precision of 0.49 ℃ (minimum precision, 0.18 ℃) within a 0.28 mm2 field of view. We demonstrated temperature mapping of multiple brain depths in head-fixed and vigorously behaving mice subjected to nociceptive stimulation, detecting lower temperatures during anesthesia compared to the awake state in ventromedial hypothalamic nucleus (VMH) at a 5 mm depth. These mappings reveal that spatio-temporal temperature distribution and its anesthesia-dependent reduction are heterogeneous rather than uniform in VMH. This endomicroscopy provides a promising platform to dissect how deviations in biophysical parameters modulate deep-brain function. |
| 書誌情報 |
Biological Sciences
発行日 2026-02
|
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.21203/rs.3.rs-8208680/v1, |
