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内容記述 |
Hybridization of carbon-based materials opens new frontiers in creating multifunctional nanosensors for biomedical and quantum sensing applications. Here, we present a novel nanosensor, CQD-FND, integrating carbon quantum dots (CQDs) and fluorescent nanodiamonds (FNDs). By utilizing a silica coating to facilitate the conjugation of FNDs and CQDs, we developed a design that effectively suppresses fluorescence quenching of CQDs caused by dangling bonds on the FND surface, enabling sufficient fluorescence measurement from both FNDs and CQDs in the hybrids. This sensor achieves ratiometric thermometry, overcoming the limitations associated with using FNDs or CQDs individually for temperature measurements. The CQD-FND sensor exhibits remarkable stability under varying environmental conditions such as ionic strength, viscosity, and pH, demonstrating robust performance in biological systems and enabling accurate temperature measurements. Moreover, we labeled FNDs with blue- or green-fluorescent CQDs, enabling multicolor labeling for magnetic resonance measurements. We successfully measured the magnetic resonance signal of CQD-FND in cells and nematode C. elegans, demonstrating its potential for multi-parameter quantum biosensing applications. These results establish CQD-FND as a versatile tool for applications ranging from bioimaging to biosensing. |
