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内容記述 |
A potent technique for crop development, induced mutagenesis enables the creation of new genetic variants. In this work, we used whole genome sequencing (WGS) for high-resolution characterization to compare mutations in rice (Oryza sativa L. cv. Nipponbare) seeds and seedlings exposed to carbon ion beams and gamma rays. Single-base substitutions (SBSs), insertions, deletions, and structural variants were among the genetic changes brought about by both irradiation treatments; however, the frequency and distribution of these changes varied greatly between the two radiation types. At comparable survival-reducing dosages in dry seed irradiation, gamma rays (2.2×10-7/bp) produced more mutations than carbon ions (1.2×10-7/bp). Dry-seed irradiation produced more mutations than seedling irradiation (1.7×10-7/bp) in case of gamma irradiation. On average for dry-seed irradiation, each gamma-ray-irradiated M2 plant had 47.4 SBSs, 29.8 deletions, and 2.5 insertions; in contrast, C-ion-irradiated mutants had fewer, with 22.3 SBSs, 20.0 deletions, and 1.1 insertions, including both homozygous and heterozygous mutations. On the other hand, equivalent gamma-ray-irradiated seedlings had comparatively lower mutations with 38.4 SBSs, 17.6 deletions, and 2.2 insertions. In the case of seed irradiation, carbon ions are approximately eight times more lethal than gamma rays per dose, and gamma ray irradiation of seedlings is approximately six times more lethal than seed irradiation from the comparative study of LD50 for survival of Nipponbare rice. |
