WEKO3
アイテム
キャピラリーゲル電気泳動を用いたニホンナシのAFLPマーカー検出技術の開発
https://repo.qst.go.jp/records/2002635
https://repo.qst.go.jp/records/2002635af2dc2b7-e41a-419b-8346-13ececdca7dd
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2026-01-27 | |||||||||||
| タイトル | ||||||||||||
| タイトル | キャピラリーゲル電気泳動を用いたニホンナシのAFLPマーカー検出技術の開発 | |||||||||||
| 言語 | ja | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
志賀 由絃
× 志賀 由絃
× 富永 晃好
× 下川 卓志
|
|||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | We developed an AFLP marker detection technique using capillary gel electrophoresis to confirm the presence or absence of mutations and to detect chimeric mutation in ion-beam irradiated Japanese pear cultivars. First, we evaluated the reliability of this method by identifying six varieties of Japanese pear. A total of 180 peaks were obtained, of which 103 were variety-specific. A phylogenetic tree based on the peaks obtained was consistent with the variety's genealogy, confirming the method's reliability. A total of 190 peaks were obtained between the ion-beam irradiated and non-irradiated individuals, of which 35 were mutant-specific peaks. Different peaks were detected from different leaves of the same individual in ion-beam irradiated plants, allowing the detection of chimeric mutation. Furthermore, the chimeric mutation rate was found to be reduced in the shoots of the two-year-old branch via the lateral buds. | |||||||||||
| 書誌情報 |
DNA多型 巻 33, 号 1, 発行日 2025-05 |
|||||||||||
| 出版者 | ||||||||||||
| 出版者 | DNA多型学会 | |||||||||||
