| アイテムタイプ |
会議発表用資料 / Presentation(1) |
| 公開日 |
2025-10-30 |
| タイトル |
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タイトル |
Effects of Carbon-Ion Beam Irradiation in Combination with Sorafenib on Hepatocellular Carcinoma Cells |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_c94f |
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資源タイプ |
conference presentation |
| 著者 |
Sai Sei
Eun Ho Kim
Suzuki Masao
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| 抄録 |
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内容記述 |
Aims In this study, we investigate the cell killing effects of carbon-ion beam IR combined with multikinase inhibitor sorafenib on hepatocellular carcinoma (HCC) cells.Materials & Method HCC cells and cancer stem cells (CSCs) sorted from HepG2 and Huh7 were treated with carbon-ion beam irradiation (IR) alone or in combination with sorafenib, a multikinase inhibitor, then cell viability, colony and spheroid formation ability, cell death- and angiogenesis-related gene expression changes were analyzed.Results The combination of carbon-ion beam IR with sorafenib (10uM) significantly suppressed HCC cell viability and remarkably decreased colony and spheroid formation ability in CSCs (CD133+/CD90+, CD44+/ESA+) isolated from HepG2 cells compared to carbon-ion beam IR or X-ray IR alone. Annexin V and caspase3/7 activity assay revealed that the combination of carbon-ion beam IR and sorafenib significantly induced apoptosis compared to that of its alone treatment. Custom PCR array system analysis showed that carbon-ion beam IR combined with sorafenib significantly enhanced expressions of Bax and Cytochrome c and decreased HIF1a and VEGF.Conclusions The combination treatment of carbon-ion beam IR and sorafenib significantly destroyed HCC cells including CSCs accompanied with apoptosis induction. |
| 会議概要（会議名, 開催地, 会期, 主催者等） |
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内容記述 |
the 68th Annual Meeting of the Japanese Radiation Research Society and the 6th Asian Congress of Radiation Research (JRRS/ACRR2025) |
| 発表年月日 |
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日付 |
2025-10-25 |
