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内容記述 |
Ion beam irradiation is one of the physical mutagen techniques for creating genetic variation in crop improvement programs. This technique has been widely used in rice breeding program. The radiosensitivity study was conducted to determine the optimum doses for ion beam irradiation on rice seedlings and to observe the biological response in terms of seedling height (cm). Two rice genotypes were used in this study: NM7 and MR220CL2. Seeds of both genotypes were irradiated at the National Institutes for Quantum Science and Technology (QST), Takasaki, Japan, at doses of 0,10,20,40,60,80 and 100 Gy respectively. The seedlings were germinated in a petri dish for 7 days and continued to grow at LED light for 14 days. The survival seedlings were transplanted at the pot in the glasshouse. and data for seedling height was collected on day 30th after germination. The result showed that the shoot length significantly decreased as dose increased. Next, the optimum doses for NM7 are around 26 to 46 Gy. Meanwhile for, MR220CL2 is around 19 to 38 Gy respectively. Further screening and selection in subsequent populations is needed to identify potential individuals and inheritance of selected traits from each generation. |
