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Repeated artificial mutagenesis during experimental evolution accelerates the acquisition of high-temperature tolerance by Bradyrhizobium diazoefficiens
https://repo.qst.go.jp/records/2001842
https://repo.qst.go.jp/records/2001842012b82df-b7ab-450c-a5b3-9560cbd17963
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2025-12-17 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Repeated artificial mutagenesis during experimental evolution accelerates the acquisition of high-temperature tolerance by Bradyrhizobium diazoefficiens | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
Hase Yoshihiro
× Hase Yoshihiro
× Nagafune Ikuko
× Sato Katsuya
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Effective mutant screening is critical for improving industrial microorganisms. This study was conducted to evaluate the effect of repeated mutagenesis on the experimental evolution of rhizobial high-temperature tolerance because the repeated mutagenesis approach is conducive to accumulating beneficial mutations relatively quickly. Wild-type Bradyrhizobium diazoefficiens USDA110 cells grow optimally at 32−34 °C, but their growth is markedly retarded at 36 °C. Wild-type cells were subcultured in a 96-well deep-well plate for 76 or 83 days, with a gradual increase in temperature from 34.0 to 37.0 °C. Additionally, they were exposed to gamma radiation (1–120 Gy, 10 times in total) during the experimental period. The 40-Gy and 80-Gy treatments generated the most lines with high-temperature-tolerance. However, after extended subculturing without mutagenesis, tolerant lines obtained following the 80-Gy treatment produced smaller colonies than tolerant lines obtained after the 40-Gy treatment, suggesting the accumulation of deleterious mutations. These results imply that approximately 40 Gy is the appropriate dose for accumulating beneficial mutations under our experimental conditions. The two most tolerant lines obtained via the 30-Gy treatment commonly had a mutation in the 16S ribosomal RNA gene and the DNA-directed RNA polymerase subunit beta′ gene (rpoC), possibly reflecting a strong relationship with high-temperature tolerance. These results suggest that experimental evolution with an appropriately increased mutation frequency is effective for obtaining novel mutants. | |||||||||||
| 書誌情報 |
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 発行日 2025-12 |
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| 出版者 | ||||||||||||
| 出版者 | Elsevier | |||||||||||
| DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1016/j.mrfmmm.2025.111919 | |||||||||||
