| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-06-05 |
| タイトル |
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タイトル |
Multi-spin Redox Sensor for Electron Paramagnetic Resonance Studies of Tissue Redox State In Vivo: Validation of Data with a Conventional Spin Probe |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Dessislava Lazarova
Biliana Nikolova
Zhivko Zhelev
Plamen Getsov
Milka Mileva
Rumiana Bakalova
青木 伊知男
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Redox imaging is one of the fastest growing areas in diagnostics of pathologies accompanied by redox imbalance. We describe a multi-spin redox sensor (RS) and its application for redox imaging in mice using EPR spectroscopy. The probe is composed of a quantum dot functionalized with a cyclodextrin shell, conjugated with nitroxide residues (TEMPO) and triphenylphosphonium to achieve intracellular delivery. The data were validated with a conventional spin probe, mito-TEMPO. RS had the same EPR contrast and significantly higher MRI contrast compared to mito-TEMPO. RS circulated longer in the bloodstream than mito-TEMPO, with both substances undergoing reduction in the blood. The distribution of RS and mito-TEMPO in different organs was equal, except for the brain. No adverse side effects were found in mice at the selected dose. Using a non-toxic nanoparticle in the chemical structure of RS as a carrier of nitroxide residues would enhance its translational relevance. |
| 書誌情報 |
In Vivo
発行日 2025-12
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| 出版者 |
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出版者 |
The International Institute of Anticancer Research |
