| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-26 |
| タイトル |
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|
タイトル |
Protocol for RNA extraction from glioblastoma cell-line-derived spheroids embedded in atelocollagen gel guided by real-time imaging. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Mayumi Fujita
Kaori Imadome
Tetsuro Sato
Hirokazu Hirakawa
Junko Kado
Asako Yamagiri
Taichi Miura
Satoshi Kamimura
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Microscopy advancements enable real-time imaging and analysis of cancer spheroids embedded in atelocollagen gel. Here, we present a protocol for extracting high-quality total RNA from 3D tumor spheroids embedded in atelocollagen gel, including spheroids and invading cells dispersed into surrounding gel. We describe steps for spheroid formation, gel embedding, live-cell imaging, and image analysis. We then detail procedures for collecting sufficient quantities of total RNA suitable for comprehensive transcriptome analysis. This approach advances transcriptome profiling in diverse 3D cultured samples. |
| 書誌情報 |
STAR protocols
巻 6,
号 2,
p. 103839,
発行日 2025-05
|
| 出版者 |
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|
出版者 |
CellPress |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2666-1667 |
| PubMed番号 |
|
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
40413748 |
| DOI |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.xpro.2025.103839 |
