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DNA sensing based on aggregation of Janus particles using dynamic light scattering
https://repo.qst.go.jp/records/2001519
https://repo.qst.go.jp/records/2001519b0fc323b-1902-46cc-9b09-4f5794a5144d
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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| 公開日 | 2025-02-27 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | DNA sensing based on aggregation of Janus particles using dynamic light scattering | |||||||||||||||
| 言語 | en | |||||||||||||||
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| 言語 | eng | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| 著者 |
Akihisa Miyagawa
× Akihisa Miyagawa
× Chisa Ito
× Ueda Yasuyuki
× Shigenori Nagatomo
× Kiyoharu Nakatani
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| 抄録 | ||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||
| 内容記述 | Background The aggregation of isotropic particles through interparticle reactions poses a challenge in control due to the ability of all surfaces to bind to each other, rendering the quantitative detection of such interparticle reactions based on particle size difficult. Here, we proposed a novel detection scheme for DNA utilizing an assembly of Janus particles (JPs) employing dynamic light scattering (DLS). DNA molecules are tethered on one hemisphere of the JP, while the other hemisphere retains its hydrophobic properties. Results Aggregation of JPs was induced by the sandwich hybridization of target DNA between them. The assembly of JPs was effectively monitored by the changes in hydrodynamic diameter detected by DLS, revealing that aggregation peaks at 2?3 particles and further reaction was hindered due to the inability of one hemisphere of the JP to interact with another JP. The target DNA demonstrated detectability at concentrations as low as several tens of pM to several nM using a digital sensing method. The two types of target DNA, such as simple (14 base pairs) and HIV-2 specific sequences (20 base pairs) were detectable at nM and pM levels, respectively. Moreover, we substantiated the robustness of our detection scheme through stoichiometric calculations based on an equilibrium model. The present detection mechanism was well explained based on the binding affinity of DNA hybridization. Significance This detection method harnesses the anisotropic nature of JPs and represents the first detection approach based on aggregation. By altering the modification molecules on JPs to match target molecules, such as proteins and organic compounds, a wide range of versatile molecules can be detected using this scheme with high sensitivity. This underscores the broad applicability of the present method. |
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| 書誌情報 |
Analytica Chimica Acta 巻 1318, 発行日 2025-02 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||
| 収録物識別子 | 1873-4324 | |||||||||||||||
| DOI | ||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||
| 関連識別子 | 10.1016/j.aca.2024.342933 | |||||||||||||||
