| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-06-02 |
| タイトル |
|
|
タイトル |
Hyperpolarized [1-13C]pyruvate NMR spectroscopy reveals transition of tumor energy metabolism in microscale multicellular spheroids |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Takakusagi Yoichi
Takakusagi Kaori
Inoue Kaori
Saito Keita
Homma Yoshimi
Ichikawa Kazuhiro
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Hyperpolarized (HP) [1-13C]pyruvate nuclear magnetic resonance (NMR) spectroscopy was employed to investigate tumor energy metabolism in microscale multicellular spheroids of a few hundred micrometers in diameter, serving as a model of early-phase tumorigenesis in vivo. A three-dimensional static culture of murine squamous cell carcinoma (SCCVII) cells formed uniform smaller multicellular spheroids (~150 μm in diameter), without hypoxic or necrotic cores, yet these spheroids exhibited resistance to anti-tumor drugs. HP [1-13C]pyruvate NMR spectroscopy of SCCVII spheroids revealed an increased conversion of pyruvate to lactate compared to monolayer cultures, indicating enhanced aerobic glycolysis in the aggregated cells. Additionally, HP spectroscopy differentiated the degree of aerobic glycolysis in human prostate tumor spheroids―DU145 (~120 μm) and PC-3 (~230 μm)―as evidenced by the upregulation of genes associated with lactate production and cellular transport. The Lac/Pyr ratio among spheroids correlated with those observed in homogenate samples of corresponding tumors grown in mice. These findings suggest that HP [1-13C]pyruvate NMR spectroscopy may serve as a metabolic biomarker for early-phase tumorigenesis in vivo. |
| 書誌情報 |
Scientific Reports
巻 in press,
発行日 2025-06
|
| 出版者 |
|
|
出版者 |
Springer Nature |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1038/s41598-025-03454-1 |
