| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-01-07 |
| タイトル |
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タイトル |
Observation of the Liquid-Liquid Phase Separation of FUS-LC Using Vacuum-Ultraviolet Circular Dichroism Spectroscopy. |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Kentaro Fujii
Yudai Izumi
Nobuo Maita
Koichi Matsuo
Masato Kato
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
To reveal the structural mechanism by which the low-complexity domain of the fused in sarcoma protein (FUS-LC) mediates liquid-liquid phase separation (LLPS), we conducted a vacuum-ultraviolet circular dichroism (VUV-CD) spectroscopic study, a technique to analyze the secondary structures of proteins. The VUV-CD measurements were performed at the BL12 VUV-CD station at the Hiroshima Synchrotron Radiation Center (HiSOR) in Japan. CD spectra were measured between 180 and 260?nm while controlling the temperature of samples from 37°C to 5°C to obtain the LLPS of FUS-LC. The CD spectrum obtained at 37°C exhibited a large negative peak at 195?nm and a small negative shoulder near 220?nm. The peak intensity around 195?nm decreased as the sample temperature decreased. The spectral changes originated from the LLPS formation. |
| 書誌情報 |
Chirality
発行日 2024-08
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| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
39129227 |
