| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-09-05 |
| タイトル |
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|
タイトル |
iPS cell generation-associated point mutations include many C?>?T substitutions via different cytosine modification mechanisms. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Ryoko Araki
Tomo Suga
Yuko Hoki
Kaori Imadome
Misato Sunayama
Satoshi Kamimura
Mayumi Fujita
Masumi Abe
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| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Genomic aberrations are a critical impediment for the safe medical use of iPSCs and their origin and developmental mechanisms remain?unknown. Here we find through WGS analysis of human and mouse iPSC lines that genomic mutations are de novo events and that, in addition to unmodified cytosine base prone to deamination, the DNA methylation sequence CpG represents a significant mutation-prone site. CGI and TSS regions show increased mutations in iPSCs and elevated mutations are observed in retrotransposons, especially in the AluY subfamily. Furthermore, increased cytosine to thymine mutations are observed in differentially methylated regions. These results indicate that in addition to deamination of cytosine, demethylation of methylated cytosine, which plays a central role in genome reprogramming, may act mutagenically during iPSC generation. |
| 書誌情報 |
Nature communications
巻 15,
号 1,
p. 4946,
発行日 2024-06
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| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2041-1723 |
| PubMed番号 |
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|
識別子タイプ |
PMID |
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関連識別子 |
38862540 |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1038/s41467-024-49335-5 |
