| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-02-22 |
| タイトル |
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タイトル |
Observation of the liquid-liquid phase separation of FUS-LC using vacuum-ultraviolet circular dichroism spectroscopy |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Fujii Kentaro
Kentaro Fujii
Izumi Yudai
Maita Nobuo
Koichi Matsuo (Hiroshima Univ.)
Kato Masato
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
To reveal the structural mechanism by which the low-complexity domain of the fused in sarcoma protein (FUS-LC) mediates liquid-liquid phase separation (LLPS), we conducted a vacuum-ultraviolet circular dichroism (VUV-CD) spectroscopic study, a technique to analyze the secondary structures of proteins. The VUV-CD measurements were performed in the BL12 VUV-CD station at the Hiroshima Synchrotron Radiation Center (HiSOR) in Japan. CD spectra were measured between 180 and 260 nm while controlling the temperature of samples from 37℃ to 5℃ to obtain the LLPS of FUS-LC. The CD spectrum obtained at 37℃ exhibited a large negative peak at 195 nm and a small negative shoulder near 220 nm. The peak intensity around 195 nm decreased as the sample temperature decreased. The spectral changes originated from the LLPS formation. |
| 書誌情報 |
Chrality
発行日 2024-07
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| 出版者 |
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出版者 |
Wiley |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1002/chir.23707 |
