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内容記述 |
While long-chain alkenone (defined as alkenone) is known to have a potential for industrial application, its biosynthetic pathway is still largely unknown. In the present study, we obtained a novel mutant named C8-14 strain altering alkenone composition with heavy-ion beam irradiation to the haptophyte Tisochrysis lutea. This mutant showed higher levels of tri-unsaturated alkenones and significantly lower amounts of alkenones and alkenoates than the wild-type cells. In addition, the total amounts of fatty acids in the neural lipid fraction of the C8-14 strain were higher than those in the wild-type cells. The primary sequence of the genes related to alkenone desaturases in the C8-14 strain, including the paralogous genes found in this study, were identical to those in the wild-type cells. However, the expression level of the putative alkenone desaturase gene was altered. The expression of the TOD-2 gene was higher in the C8-14 cells than in the wild-type cells. This is the first report, to the best of our knowledge, to create the mutant with the altered unsaturation of alkenones. |
