| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-03-08 |
| タイトル |
|
|
タイトル |
Micro- and nanochamber array system for single enzyme assays. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kazuki Iijima
Noritada Kaji
Manabu Tokeshi
Yoshinobu Baba
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Arrays of small reaction containers, ranging from 624 femtoliters (10?L) to 270 attoliters (10?L), for capturing a single enzyme molecule and measuring the activity were developed along with a new reversible sealing system based on a pneumatic valve actuator made of polydimethylsiloxane (PDMS). The valve was actuated by PBS solution, effectively preventing evaporation of the solution from the micro- and nanochambers and allowing the assay to be performed over a long period of time. The hydrolysis rates of β-D-galactosidase (β-gal), k, were decreased according to the decrease of the chamber size, and the overall tendency seems to be symmetrically related to the specific surface area of the chambers even under the prevented condition of non-specific adsorption. The spatial localization of the protons in the chambers, which might could affect the dissociation state of the proteins, was also investigated to explain the decrease in the hydrolysis rate. The developed chamber system developed here may be useful for artificially reproducing the confined intracellular environment and molecular crowding conditions. |
| 書誌情報 |
Scientific reports
巻 13,
号 1,
p. 13322,
発行日 2023-08
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2045-2322 |
| PubMed番号 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
37587179 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1038/s41598-023-40544-4 |
