| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-02-29 |
| タイトル |
|
|
タイトル |
Label-free mid-infrared photothermal live-cell imaging beyond video rate. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Genki Ishigane
Keiichiro Toda
Miu Tamamitsu
Hiroyuki Shimada
Venkata Ramaiah Badarla
Takuro Ideguchi
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Advancement in mid-infrared (MIR) technology has led to promising biomedical applications of MIR spectroscopy, such as liquid biopsy or breath diagnosis. On the contrary, MIR microscopy has been rarely used for live biological samples in an aqueous environment due to the lack of spatial resolution and the large water absorption background. Recently, mid-infrared photothermal (MIP) imaging has proven to be applicable to 2D and 3D single-cell imaging with high spatial resolution inherited from visible light. However, the maximum measurement rate has been limited to several frames s, limiting its range of use. Here, we develop a significantly improved wide-field MIP quantitative phase microscope with two orders-of-magnitude higher signal-to-noise ratio than previous MIP imaging techniques and demonstrate live-cell imaging beyond video rate. We first derive optimal system design by numerically simulating thermal conduction following the photothermal effect. Then, we develop the designed system with a homemade nanosecond MIR optical parametric oscillator and a high full-well-capacity image sensor. Our high-speed and high-spatial-resolution MIR microscope has great potential to become a new tool for life science, in particular for live-cell analysis. |
| 書誌情報 |
Light, science & applications
巻 12,
号 1,
p. 174,
発行日 2023-07
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2047-7538 |
| PubMed番号 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
37463888 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1038/s41377-023-01214-2 |
