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Two-photon optogenetics-based assessment of neuronal connectivity in healthy and chronic hypoperfusion mice
https://repo.qst.go.jp/records/2001051
https://repo.qst.go.jp/records/200105112cd002a-8176-47a0-85c1-b9e7f4b09fd2
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| 公開日 | 2025-03-14 | |||||||||||||||||
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| タイトル | Two-photon optogenetics-based assessment of neuronal connectivity in healthy and chronic hypoperfusion mice | |||||||||||||||||
| 言語 | en | |||||||||||||||||
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| 言語 | eng | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 著者 |
Yoshioka Masaki
× Yoshioka Masaki
× Takahashi Manami
× Jeffrey Kershaw
× Handa Mariko
× Takada Ayaka
× Takuwa Hiroyuki
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Significance: Two-photon optogenetics and simultaneous calcium imaging can be used to visualize the response of surrounding neurons with respect to the activity of an optically stimulated target neuron, providing a direct method to assess neuronal connectivity. Aim: Develop a two-photon optogenetics-based method for evaluating neuronal connectivity, compare it to the existing indirect resting-state synchrony method, and investigate application of the method to brain pathophysiology. Approach: C1V1-mScarlet was introduced into GCaMP6s-expressing transgenic mice with an adeno-associated virus. Optical stimulation of a single target neuron and simultaneous calcium imaging of the target and surrounding cells were performed. Neuronal connectivity was evaluated from the correlation between the fluorescence intensity of the target and surrounding cells. Results: Neuronal connectivity in living brain was evaluated using two-photon optogenetics. However, resting-state synchrony was not always consistent with two-photon optogenetics-based connectivity. Comparison with neuronal synchrony measured during sensory stimulation suggested that the disagreement was due to external sensory input. Two-photon optogenetics-based connectivity significantly decreased in the common carotid artery occlusion model, while there was no significant change in the control group. Conclusions: We successfully developed a direct method to evaluate neuronal connectivity in living brain using two-photon optogenetics. The technique was successful in detecting connectivity impairment in hypoperfusion model mice. |
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| 書誌情報 |
Neurophotonics 巻 11, 号 3, 発行日 2024-09 |
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| DOI | ||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | 10.1117/1.NPh.11.3.035009 | |||||||||||||||||
