| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-02-13 |
| タイトル |
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タイトル |
Resistive pulse sensing on a capillary-assisted microfluidic plat-form for on-site single particle analyses. |
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言語 |
ja |
| 言語 |
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言語 |
jpn |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Taisuke Shimada
Keiko Fujino
安井 隆雄
Noritada Kaji
植田 泰之
藤井 健太郎
湯川 博
馬場 嘉信
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Capillary-assisted flow is valuable for utilizing microfluidics-based electrical sensing platforms at on-site locations by simplifying microfluidic operations and system construction; however, incorporating capillary-assisted flow in platforms requires easy microfluidic modification and stability over time for capillary-assisted flow generation and sensing performance. Herein, we report a capillary-assisted microfluidics-based electrical sensing platform using a one-step modification of polydimethylsiloxane (PDMS) with polyethylene glycol (PEG). As a model of electrical sensing platforms, this work focused on resistive pulse sensing (RPS) using a micropore in a microfluidic chip for label-free electrical detection of single analytes, and filling the micropore with an electrolyte is the first step to perform this RPS. The PEG?PDMS surfaces remained hydrophilic after ambient storage for 30 d and assisted in generating an electrolyte flow for filling the micropore with the electrolyte. We demonstrated the successful detection and size analysis of micrometer particles and bacterial cells based on RPS using the microfluidic chip stored in a dry state for 30 d. Combining this capillary-assisted microfluidic platform with a portable RPS system makes on-site detection and analysis of single pathogens possible. |
| 書誌情報 |
Analytical Chemistry
巻 95,
号 50,
p. 18335-18343,
発行日 2023-12
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| 出版者 |
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出版者 |
American Chemical Society |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1021/acs.analchem.3c02539 |
