| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-03-13 |
| タイトル |
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|
タイトル |
Assessing the applicability of a modified replication banding protocol for the analysis of radiation-induced chromosomal aberrations in cultured human lymphocytes |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Akiyama Miho
Tominaga Takako
Takashima Yoshio
Ishii Kotaro
Suto Yumiko
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
To analyze radiation-induced chromosomal aberrations in cultured human peripheral blood lymphocytes, a modified protocol for replication banding in combination with fluorescence in situ hybridization (FISH) using whole-chromosome-specific DNA probes was examined. The aim of the major modification of the experimental procedure was to efficiently generate clear and consistent replication bands along metaphase chromosomes in the first division of lymphocytes after culture initiation. This technique enabled the mapping of the sites of rearrangements directly on banded chromosomes at 400?550 band levels. On the basis of the characteristics of replication banding, the procedure described in the present study demonstrated the applicability for the analysis of gamma-ray-induced chromosomal aberrations in early- and late-replicating X chromosomes in cultured human peripheral blood lymphocytes. |
| 書誌情報 |
Cytologia
巻 89,
号 1,
p. 1-8,
発行日 2024-03
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1348-7019 |
