| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-06-12 |
| タイトル |
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タイトル |
Pimonidazole-alkyne conjugate for sensitive detection of hypoxia by Cu-catalyzed click reaction |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Tamura Iori
Sakamoto M. Daichi
Yi Bo
Saito Yutaro
Yamada Naoki
Takakusagi Yoichi
Sando Shinsuke
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Hypoxia is involved in various diseases, such as cancers. Pimonidazole has often been used as the gold-standard marker to visualize hypoxic regions. Pimonidazole labels hypoxic regions by forming a covalent bond with a neighboring protein under hypoxic conditions in the body, which is detected by immunohistochemistry performed on tissue sections. To date, some Pimonidazole-fluorophore conjugates have been reported as fluorescent probes for hypoxia imaging that do not require immunostaining. They are superior to Pimonidazole because immunostaining can produce high background signals. However, large fluorophores in the conjugates may alter the original biodistribution and reactivity. Here, we report a new hypoxia marker, Pimo-yne, as a Pimonidazole-alkyne conjugate. Pimo-yne has a similar hypoxia detection capability as Pimonidazole because the alkyne tag is small and can be detected by Cu-catalyzed click reaction with azide-tagged fluorescent dyes. We successfully visualized hypoxic regions in tumor tissue sections using Pimo-yne with reduced background signals. The detected regions overlapped well with those detected by Pimonidazole immunohistochemistry. To further reduce the background, we employed a turn-on azide-tagged fluorescent dye. |
| 書誌情報 |
Analytical Sciences
巻 40,
p. 1061-1070,
発行日 2024-03
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| 出版者 |
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出版者 |
Springer Nature |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1348-2246 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1007/s44211-024-00520-y |
