| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-01-08 |
| タイトル |
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|
タイトル |
A mosaic adeno-associated virus vector as a versatile tool that exhibits high levels of transgene expression and neuron specificity in primate brain. |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kei Kimura
Yuji Nagai
Gaku Hatanaka
Yang Fang
Soshi Tanabe
Andi Zheng
Maki Fujiwara
Mayuko Nakano
Yukiko Hori
Ryosuke F Takeuchi
Mikio Inagaki
Takafumi Minamimoto
Ichiro Fujita
Ken-Ichi Inoue
Masahiko Takada
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Recent emphasis has been placed on gene transduction mediated through recombinant adeno-associated virus (AAV) vector to manipulate activity of neurons and their circuitry in the primate brain. In the present study, we created a novel vector of which capsid was composed of capsid proteins derived from both of the AAV serotypes 1 and 2 (AAV1 and AAV2). Following the injection into the frontal cortex of macaque monkeys, this mosaic vector, termed AAV2.1 vector, was found to exhibit the excellence in transgene expression (for AAV1 vector) and neuron specificity (for AAV2 vector) simultaneously. To explore its applicability to chemogenetic manipulation and in vivo calcium imaging, the AAV2.1 vector expressing excitatory DREADDs or GCaMP was injected into the striatum or the visual cortex of macaque monkeys, respectively. Our results have defined that such vectors secure intense and stable expression of the target proteins and yield conspicuous modulation and imaging of neuronal activity. |
| 書誌情報 |
Nature communications
巻 14,
p. 4762,
発行日 2023-08
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| 出版者 |
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出版者 |
Springer Nature |
| ISSN |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2041-1723 |
| PubMed番号 |
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|
識別子タイプ |
PMID |
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関連識別子 |
37553329 |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1038/s41467-023-40436-1 |
