| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-12-27 |
| タイトル |
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タイトル |
Whole-Body and Whole-Organ 3D Imaging of Hypoxia Using an Activatable Covalent Fluorescent Probe Compatible with Tissue Clearing |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Sakamoto M. Daichi
Tamura Iori
Yi Bo
Hasegawa Sho
Saito Yutaro
Yamada Naoki
Takakusagi Yoichi
Kubota Shimpei
Kobayashi Minoru
Harada Hiroshi
Hanaoka Kenjiro
Taki Masayasu
Nangaku Masaomi
Tainaka Kazuki
Sando Shinsuke
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Elucidation of biological phenomena requires imaging of microenvironments in vivo. Although the seamless visualization of in vivo hypoxia from the level of whole-body to single-cell has a great potential to discover unknown phenomena in biological and medical fields, no methodology for achieving it has been established thus far. Here, we for the first time report the whole-body and whole-organ imaging of hypoxia, an important microenvironment, at single-cell resolution using activatable covalent fluorescent probes compatible with tissue clearing. We initially focused on overcoming the incompatibility of fluorescent dyes and refractive index matching solutions (RIMSs), which has greatly hindered the development of fluorescent molecular probes in the field of tissue clearing. The fluorescent dyes compatible with RIMS were then incorporated into the development of activatable covalent fluorescent probes for hypoxia. We combined the probes with tissue clearing, achieving comprehensive single-cell-resolution imaging of hypoxia in a whole mouse body and whole organs. |
| 書誌情報 |
ACS Nano
巻 18,
号 6,
p. 5167-5179,
発行日 2024-02
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| 出版者 |
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出版者 |
ACS Publications |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1936-086X |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
38301048 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1021/acsnano.3c12716 |
