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Development of Non-Electrically Controlled SalivaDirect LAMP (NEC-SD-LAMP), a New Nonelectrical Infectious Disease Testing Method
https://repo.qst.go.jp/records/2000708
https://repo.qst.go.jp/records/2000708ff9ef9e9-9704-486b-99cf-45b6888ec172
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-11-11 | |||||||||
| タイトル | ||||||||||
| タイトル | Development of Non-Electrically Controlled SalivaDirect LAMP (NEC-SD-LAMP), a New Nonelectrical Infectious Disease Testing Method | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
Kimura Yusuke
× Kimura Yusuke
× Masashi Ikeuchi
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | In this study, non-electrically controlled SalivaDirect loop-mediated isothermal amplification (NEC-SD-LAMP), which can detect infections by amplifying viral DNA expression in saliva without using electrical control systems, was developed. By this method, only by adding water to the device, viral DNA was extracted from saliva using SalivaDirect, the extracted DNA was amplified via loop-mediated isothermal amplification (LAMP), and the results were visually confirmed. Melting palmitic acid maintained the optimal temperature for the LAMP reaction, as the temperature of palmitic acid is maintained at 62.9 °C, its melting point. By exploiting the proximity of the melting point to the optimal temperature for LAMP, LAMP can be performed without electricity. We detected several viruses in the saliva using this method. NEC-SD-LAMP could clearly distinguish 3 types of viral DNA, indicating the high specificity of this reaction. Furthermore, the viral concentration detection limit of the device was 2 copies per μL, indicating that it is possible to detect DNA viral infections in saliva even before the onset of viral infection. | |||||||||
| 書誌情報 |
scientific reports 巻 13, p. 11791, 発行日 2023-07 |
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| 出版者 | ||||||||||
| 出版者 | Springer Nature | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 2045-2322 | |||||||||
| DOI | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1038/s41598-023-38800-8 | |||||||||
