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A single mutation Asp43Arg was increased 2.5-fold the catalytic activity and maintained the stability of cold-adapted endo-1,4-beta glucanase (Ef-EG2) from Eisenia fetida
https://repo.qst.go.jp/records/2000584
https://repo.qst.go.jp/records/2000584ffa39303-fe9a-40fd-ac10-e31ea1c89bfb
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| 公開日 | 2024-08-09 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | A single mutation Asp43Arg was increased 2.5-fold the catalytic activity and maintained the stability of cold-adapted endo-1,4-beta glucanase (Ef-EG2) from Eisenia fetida | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 著者 |
Kuroki Chikako
× Kuroki Chikako
× Hirano Yuu
× Nakazawa Mami
× Sakamoto Tatsuji
× Tamada Taro
× Ueda Mitsuhiro
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | To improve the specific activity at low temperatures of Ef-EG2 and to maintain thermostability, five mutant enzymes (K273R, N372D, Q387E, N402D, D43R) were produced. The two mutant enzymes (K273R, N402D) lost the cellulase activity. The specific activities of mutant N372D, Q387E, and D43R enzymes were 2.5-fold higher than that of WT enzyme over various temperatures. The denaturation temperatures (Tm) of WT and N372D, Q387E, and D43R enzymes were 55.1℃, 51.5℃, 51.2℃, and 55.6℃. D43R showed almost the same thermostability as the WT enzyme. The three-dimensional structure of D43R was not significantly different from that of the WT enzyme, but the D43R enzyme lost the ability to bind sodium ions. D43R was introduced the additional electrostatic interaction with Asp55. | |||||||||||||||||
| 書誌情報 |
Current Research in Biotechnology 巻 5, p. 100126, 発行日 2023-04 |
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| 出版者 | ||||||||||||||||||
| 出版者 | Elsevier | |||||||||||||||||
| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 2590-2628 | |||||||||||||||||
| DOI | ||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | 10.1016/j.crbiot.2023.100126 | |||||||||||||||||
