| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-07-01 |
| タイトル |
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タイトル |
Leukocyte Depletion and Size-Based Enrichment of Circulating Tumor Cells Using a Pressure-Sensing Microfiltration Device |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Daisuke Onoshima
Tetsunari Hase
Naoto Kihara
Daiki Kuboyama
Hiromasa Tanaka
Naoya Ozawa
Yukawa Hiroshi
Mitsuo Sato
Kenji Ishikawa
Yoshinori Hasegawa
Makoto Ishii
Masaru Hori
Baba Yoshinobu
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Considering the challenges in isolating circulating tumor cells (CTCs) pertaining to cellular stress and purity, we report the application of a blood microfiltration device as an optimal approach for noninvasive liquid biopsy to target CTCs. We experimentally analyzed the filtration behavior of the microfilter using pressure sensing to separate tumor cells from leukocytes in whole blood. This approach achieved an average recovery of >96% of the spiked tumor cells and depletion of >99% of total leukocytes. Furthermore, we carried out genomic profiling of the CTCs using the blood microfiltration device. The method was also applied in a clinical setting; DNA amplification was performed using a small number of microfiltered CTCs and epidermal growth factor receptor mutations were successfully detected to characterize the efficacy of molecularly targeted drugs against lung cancer. Overall, the proposed method can provide a tool for evaluating efficient filtration pressure to concentrate CTCs from whole blood. |
| 書誌情報 |
ACS Measurement Science Au
巻 3,
号 2,
p. 113-119,
発行日 2022-12
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| 出版者 |
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出版者 |
ACS Publications |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2694-250X |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1021/acsmeasuresciau.2c00057 |
