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RENEB Inter-Laboratory Comparison 2021: The Dicentric Chromosome Assay.
https://repo.qst.go.jp/records/2000452
https://repo.qst.go.jp/records/2000452cdb5b00e-3a44-4f60-8afd-25ede3798d7e
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2024-05-17 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | RENEB Inter-Laboratory Comparison 2021: The Dicentric Chromosome Assay. | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 著者 |
Endesfelder
× Endesfelder
× D. et. al.
× Akiyama Miho
× Suto Yumiko
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Purpose: After large-scale radiation accidents where many individuals are suspected to be exposed to ionizing radiation, biological and physical retrospective dosimetry assays can be important tools to aid clinical decision making by categorizing individuals into not or lowly exposed, moderately exposed or highly exposed groups. Quality-controlled inter-laboratory comparisons of simulated real accident situations are regularly performed in the frame of the European legal association RENEB (Running the European Network of Biological and Physical retrospective Dosimetry), to optimize international networking for retrospective dosimetry in case of large-scale events. Materials and methods: 33 laboratories from 22 countries participated in the current RENEB ILC (2021) for the dicentric chromosome assay. The study design included the irradiation of blood samples, blood shipment, sample processing, analysis of chromosome aberrations and dose assessment. Blood was irradiated in vitro with X-rays (240 kVp, 13 mA, ~75 keV, 1 Gy/min) to simulate an acute, homogeneous whole-body exposure. Three blind coded blood samples (sample #1: 0 Gy, sample #2: 1.2 Gy, sample #3: 3.5 Gy) were sent to each participant and the task was to culture samples, to prepare slides and to assess radiation doses based on the observed dicentric yields from 50 manually or 150 semi-automatically scored metaphases (triage mode scoring). Results: Most participants applied calibration curves from irradiations with γ-rays (67%) and some from irradiations with X-rays (24%) with variable voltages. The categorization of the blind coded samples in clinically relevant groups corresponding to individuals that were not or lowly (0-1 Gy), moderately (1-2 Gy) or highly exposed (>2 Gy) was successfully performed by all participants for sample #1 and #3 and by ?74% for sample #2. However, while most participants estimated a dose of exactly 0 Gy for the sham irradiated sample, the precise dose estimates of the samples irradiated with doses >0 Gy were systematically higher than the corresponding reference doses and showed a median deviation of 0.5 Gy (sample #2) and 0.95 Gy (sample #3) for manual scoring. By converting doses estimated based on γ-ray calibration curves to X-ray doses of a comparable mean photon energy as used in this exercise, the median deviation decreased to 0.27 Gy (sample #2) and 0.6 Gy (sample #3). Conclusions: The main aim of biological dosimetry in the case of a large-scale event is the categorization of individuals into clinically relevant groups, to aid clinical decision making. This task was successfully performed by almost all participants. Due to the accuracy of the dicentric chromosome assay and the high number of participating laboratories, a systematic shift of the dose estimates could be revealed. Differences in radiation quality (X-ray vs γ-ray) between the blind coded samples and the applied dose effect curves can probably partly explain the systematic shift. There might be several additional reasons for the observed bias (e.g. donor effects, transport, experimental conditions or the irradiation setup) and the analysis of these reasons provides great opportunities for future research. The participation of laboratories from countries around the world gave the opportunity to compare the results on an international level. |
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| 書誌情報 |
Radiation Research 巻 199, 号 6, p. 556, 発行日 2023-01 |
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| 出版者 | Radiation Research Society | |||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 1938-5404 | |||||||||||||
| DOI | ||||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | 10.1667/RADE-22-00202.1 | |||||||||||||
