| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-01-26 |
| タイトル |
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タイトル |
Feasibility study of direct CT lymphangiography in mice: comparison with interstitial CT/MR lymphangiography. |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Haruto Sugawara
Toshihiro Furuta
Akira Sumiyoshi
Megumi Iiyama
Masaru Kamitani
Aiko Suzuki
Arao Murakami
Osamu Abe
Ichio Aoki
Hiroyuki Akai
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Objectives: To establish a CT lymphangiography method in mice via direct lymph node puncture.
Methods: We injected healthy mice (n = 8) with 50 μl of water-soluble iodine contrast agent (iomeprol; iodine concentration, 350 mg/mL) subcutaneously into the left-rear foot pad (interstitial injection) and 20 μl of the same contrast agent directly into the popliteal lymph node (direct puncture) 2 days later. Additionally, we performed interstitial MR lymphangiography on eight mice as a control group. We calculated the contrast ratio for each lymph node and visually assessed the depiction of lymph nodes and lymphatic vessels on a three-point scale.
Results: The contrast ratios of 2-min post-injection images of sacral and lumbar-aortic lymph nodes were 20.7 ± 16.6 (average ± standard deviation) and 17.1 ± 12.0 in the direct puncture group, which were significantly higher than those detected in the CT or MR interstitial lymphangiography groups (average, 1.8-3.6; p = 0.008-0.019). The visual assessment scores for sacral lymph nodes, lumbar-aortic lymph nodes, and cisterna chyli were significantly better in the direct puncture group than in the CT interstitial injection group (p = 0.036, 0.009 and 0.001, respectively). The lymphatic vessels between these structures were significantly better scored in direct puncture group than in the CT or MR interstitial lymphangiography groups at 2 min after injection (all p ? 0.05).
Conclusions: In CT lymphangiography in mice, the direct lymph node puncture provides a better delineation of the lymphatic pathways than the CT/MR interstitial injection method.
Key points: ? The contrast ratios of 2-min post-injection images in the direct CT lymphangiography group were significantly higher than those of CT/MR interstitial lymphangiography groups. ? The visibility of lymphatic vessels in subjective analysis in the direct CT lymphangiography group was significantly better in the direct puncture group than in the CT/MR interstitial lymphangiography groups. ? CT lymphangiography with direct lymph node puncture can provide excellent lymphatic delineation with contrast being maximum at 2 min after injection.
Keywords: Contrast media; Lymphography; Mice; X-ray microtomography. |
| 書誌情報 |
European radiology
巻 33,
p. 5028-5036,
発行日 2023-01
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| 出版者 |
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出版者 |
Springer Nature |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1432-1084 |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
36719498 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1007/s00330-023-09423-4 |
