| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-09-08 |
| タイトル |
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タイトル |
Dynamic imaging analysis reveals Auger electron-emitting radio-cisplatin induces DNA damage depending on the cell cycle |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Obata Honoka
Akihiro Kurimasa
Tadanori Muraoka
Tsuji Atsushi
Kondo Katsuya
Kuwahara Yoshikazu
Minegishi Katsuyuki
Nagatsu Kotaro
Ogawa Mikako
Zhang Ming-Rong
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Auger electrons can induce nanoscale physiochemical damage to DNA. The present study reports a sequential and systematic evaluation of the relationship between DNA damage such as double-strand breaks (DSBs) and the cell cycle
for the Auger electron-emitting agent radiolabeled cisplatin with DNA binding ability. For dynamic imaging analysis, we used U2OS-derived cancer cells expressing two fluorescent fusion proteins: tumor-suppressor p53 binding protein 1 with a green fluorescent protein (53BP1-EGFP) and proliferating cell nuclear antigen with a red fluorescent protein (PCNA-DsRed). Time-lapse images of the cells were quantitatively analyzed using the ImageJ software with
the deepImageJ plugin and the Google Colaboratory platform. From the middle-to-late G1 phase, around the G1-to-S phase transition, we found increased 53BP1 foci in cells treated with the radio-cisplatin. The radio-cisplatin caused significantly more DSBs than the nonradioactive cisplatin and saline in the G1 phase but not in the other phases. These results indicate that Auger electron-induced DNA damage, including DSBs, depends on the cell cycle. The G1 phase, which is associated with low DNA repair capacity and high radiosensitivity, is a promising target; thus, combining radiolabeled cisplatin with agents that arrest cells in the G1 phase could improve the DNA-damaging effect of Auger electrons and their therapeutic efficacy. |
| 書誌情報 |
Biochemical and Biophysical Research Communications
巻 637,
p. 286,
発行日 2022-12
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0006-291X |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
36410278 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.bbrc.2022.11.016 |
