| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-01-25 |
| タイトル |
|
|
タイトル |
Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells ant neuronal progenitor cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Mikio Shimada
Takumi Tokumiya
Tomoko Miyake
Kaima Tsukada
Norie Kanzaki
Yanagihara Hiromi
Junya Kobayashi
Yoshihisa Matsumoto
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Pluripotent stem cells (PSCs) have the potential to differentiate to any of the other organs. The genome DNA integrity of PSCs is maintained by a high level of transcription for a number of genes involved in DNA repair, cell cycle, and apoptosis. However, it remains unclear how high the frequency of genetic mutation is and how these DNA repair factors function in PSCs. In this study, we employed Sup F assay for the measurement of mutation frequency after UV-C irradiation in induced pluripotent stem cells (iPSCs) as PSC models and neural progenitor cells (NPCs) were derived from iPSCs as differentiated cells. iPSCs and NPCs exhibited a lower mutation frequency compared with the original skin fibroblasts. In RNA-seq analysis, iPSCs and NPCs showed a high expression of RAD18, which is involved in trans-lesion synthesis (TLS) for the emergency tolerance system during the replication process of DNA. Although RAD18 is involved in both error free and error prone TLS in somatic cells, it still remains unknown the function of RAD18 in PSCs. In this study we depleted of the RAD18 by siRNA knockdown resulted in decreased frequency of mutation in iPSCs and NPCs. Our results will provide information on the genome maintenance machinery in PSCs. |
|
言語 |
en |
| 書誌情報 |
Journal of Radiation Research
巻 64,
p. 345-351,
発行日 2024-03
|
| 出版者 |
|
|
出版者 |
Oxford University Press |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1349-9157 |
| PubMed番号 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
36634340 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1093/jrr/rrac099 |
