WEKO3
アイテム
{"_buckets": {"deposit": "2e818046-ee7b-4db2-9c4d-807f5274f754"}, "_deposit": {"created_by": 1, "id": "84322", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "84322"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00084322", "sets": ["1"]}, "author_link": ["1018456", "1018459", "1018457", "1018453", "1018461", "1018463", "1018455", "1018460", "1018462", "1018454", "1018458"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2021-03", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "5259", "bibliographicVolumeNumber": "11", "bibliographic_titles": [{"bibliographic_title": "Scientific Reports"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Cetuximab, an anti-epidermal growth factor receptor (EGFR) monoclonal antibody, is an efficient anti-tumor therapeutic agent that inhibits the activation of EGFR; however, data related to the cellular effects of prolonged cetuximab treatment are limited. In this study, the long-term cellular outcome of prolonged cetuximab treatment and the related molecular mechanism were explored in a head and neck squamous cell carcinoma cell line constitutively expressing a fluorescent ubiquitination-based cell cycle indicator. Fluorescent time-lapse imaging was used to assess clonal growth, cell motility, and cell-cycle progression. Western blot analysis was performed to measure the level of phosphorylation and protein-expression following cetuximab treatment. Over 5 days cetuximab treatment decreased cell motility and enhanced G1 phase cell arrest in the central region of the colonies. Significantly decreased phosphorylation of retinoblastoma, Skp2, and Akt-mTOR proteins, accumulation of p27Kip1, and induction of type II LC3B were observed over 8 days cetuximab treatment. Results of the present study elucidate the cetuximab-dependent inhibition of cell migration, resulting in high cell density-related stress and persistent cell-cycle arrest at G1 phase culminating in autophagy. These findings provide novel molecular insights related to the anti-tumor effects of prolonged cetuximab treatment with the potential to improve future therapeutic strategy.", "subitem_description_type": "Abstract"}]}, "item_8_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nature"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1038/s41598-021-84877-4", "subitem_relation_type_select": "DOI"}}]}, "item_8_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.nature.com/articles/s41598-021-84877-4", "subitem_relation_type_select": "URI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2045-2322", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Okuyama, Kohei"}], "nameIdentifiers": [{"nameIdentifier": "1018453", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Keiji, Suzuki"}], "nameIdentifiers": [{"nameIdentifier": "1018454", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Naruse, Tomofumi"}], "nameIdentifiers": [{"nameIdentifier": "1018455", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsuchihashi, Hiroki"}], "nameIdentifiers": [{"nameIdentifier": "1018456", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yanamoto, Souichi"}], "nameIdentifiers": [{"nameIdentifier": "1018457", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kaida, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "1018458", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miura, Masahiko"}], "nameIdentifiers": [{"nameIdentifier": "1018459", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Umeda, Masahiro"}], "nameIdentifiers": [{"nameIdentifier": "1018460", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shunichi, Yamashita"}], "nameIdentifiers": [{"nameIdentifier": "1018461", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Keiji, Suzuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1018462", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shunichi, Yamashita", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1018463", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Prolonged cetuximab treatment promotes p27Kip1-mediated G1 arrest and autophagy in head and neck squamous cell carcinoma", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Prolonged cetuximab treatment promotes p27Kip1-mediated G1 arrest and autophagy in head and neck squamous cell carcinoma"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/84322", "pubdate": {"attribute_name": "公開日", "attribute_value": "2021-09-30"}, "publish_date": "2021-09-30", "publish_status": "0", "recid": "84322", "relation": {}, "relation_version_is_last": true, "title": ["Prolonged cetuximab treatment promotes p27Kip1-mediated G1 arrest and autophagy in head and neck squamous cell carcinoma"], "weko_shared_id": -1}
Prolonged cetuximab treatment promotes p27Kip1-mediated G1 arrest and autophagy in head and neck squamous cell carcinoma
https://repo.qst.go.jp/records/84322
https://repo.qst.go.jp/records/8432294e1ae32-ca06-4642-8e0c-ba28828cd011
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2021-09-30 | |||||
タイトル | ||||||
タイトル | Prolonged cetuximab treatment promotes p27Kip1-mediated G1 arrest and autophagy in head and neck squamous cell carcinoma | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Okuyama, Kohei
× Okuyama, Kohei× Keiji, Suzuki× Naruse, Tomofumi× Tsuchihashi, Hiroki× Yanamoto, Souichi× Kaida, Atsushi× Miura, Masahiko× Umeda, Masahiro× Shunichi, Yamashita× Keiji, Suzuki× Shunichi, Yamashita |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cetuximab, an anti-epidermal growth factor receptor (EGFR) monoclonal antibody, is an efficient anti-tumor therapeutic agent that inhibits the activation of EGFR; however, data related to the cellular effects of prolonged cetuximab treatment are limited. In this study, the long-term cellular outcome of prolonged cetuximab treatment and the related molecular mechanism were explored in a head and neck squamous cell carcinoma cell line constitutively expressing a fluorescent ubiquitination-based cell cycle indicator. Fluorescent time-lapse imaging was used to assess clonal growth, cell motility, and cell-cycle progression. Western blot analysis was performed to measure the level of phosphorylation and protein-expression following cetuximab treatment. Over 5 days cetuximab treatment decreased cell motility and enhanced G1 phase cell arrest in the central region of the colonies. Significantly decreased phosphorylation of retinoblastoma, Skp2, and Akt-mTOR proteins, accumulation of p27Kip1, and induction of type II LC3B were observed over 8 days cetuximab treatment. Results of the present study elucidate the cetuximab-dependent inhibition of cell migration, resulting in high cell density-related stress and persistent cell-cycle arrest at G1 phase culminating in autophagy. These findings provide novel molecular insights related to the anti-tumor effects of prolonged cetuximab treatment with the potential to improve future therapeutic strategy. | |||||
書誌情報 |
Scientific Reports 巻 11, p. 5259, 発行日 2021-03 |
|||||
出版者 | ||||||
出版者 | Nature | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2045-2322 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/s41598-021-84877-4 | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.nature.com/articles/s41598-021-84877-4 |