{"created":"2023-05-15T15:02:10.671589+00:00","id":84322,"links":{},"metadata":{"_buckets":{"deposit":"2e818046-ee7b-4db2-9c4d-807f5274f754"},"_deposit":{"created_by":1,"id":"84322","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"84322"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00084322","sets":["1"]},"author_link":["1018456","1018459","1018457","1018453","1018461","1018463","1018455","1018460","1018462","1018454","1018458"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5259","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cetuximab, an anti-epidermal growth factor receptor (EGFR) monoclonal antibody, is an efficient anti-tumor therapeutic agent that inhibits the activation of EGFR; however, data related to the cellular effects of prolonged cetuximab treatment are limited. In this study, the long-term cellular outcome of prolonged cetuximab treatment and the related molecular mechanism were explored in a head and neck squamous cell carcinoma cell line constitutively expressing a fluorescent ubiquitination-based cell cycle indicator. Fluorescent time-lapse imaging was used to assess clonal growth, cell motility, and cell-cycle progression. Western blot analysis was performed to measure the level of phosphorylation and protein-expression following cetuximab treatment. Over 5 days cetuximab treatment decreased cell motility and enhanced G1 phase cell arrest in the central region of the colonies. Significantly decreased phosphorylation of retinoblastoma, Skp2, and Akt-mTOR proteins, accumulation of p27Kip1, and induction of type II LC3B were observed over 8 days cetuximab treatment. Results of the present study elucidate the cetuximab-dependent inhibition of cell migration, resulting in high cell density-related stress and persistent cell-cycle arrest at G1 phase culminating in autophagy. These findings provide novel molecular insights related to the anti-tumor effects of prolonged cetuximab treatment with the potential to improve future therapeutic strategy.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-021-84877-4","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s41598-021-84877-4","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okuyama, Kohei"}],"nameIdentifiers":[{"nameIdentifier":"1018453","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Keiji, Suzuki"}],"nameIdentifiers":[{"nameIdentifier":"1018454","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Naruse, Tomofumi"}],"nameIdentifiers":[{"nameIdentifier":"1018455","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuchihashi, Hiroki"}],"nameIdentifiers":[{"nameIdentifier":"1018456","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanamoto, Souichi"}],"nameIdentifiers":[{"nameIdentifier":"1018457","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kaida, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"1018458","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miura, Masahiko"}],"nameIdentifiers":[{"nameIdentifier":"1018459","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Umeda, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"1018460","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shunichi, Yamashita"}],"nameIdentifiers":[{"nameIdentifier":"1018461","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Keiji, Suzuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1018462","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shunichi, Yamashita","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1018463","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Prolonged cetuximab treatment promotes p27Kip1-mediated G1 arrest and autophagy in head and neck squamous cell carcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Prolonged cetuximab treatment promotes p27Kip1-mediated G1 arrest and autophagy in head and neck squamous cell carcinoma"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-09-30"},"publish_date":"2021-09-30","publish_status":"0","recid":"84322","relation_version_is_last":true,"title":["Prolonged cetuximab treatment promotes p27Kip1-mediated G1 arrest and autophagy in head and neck squamous cell carcinoma"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:30:21.278196+00:00"}