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X-ray Structural Study on Polyketide Synthase Involved in Olivetolic Acid Biosynthesis in Cannabis sativa
https://repo.qst.go.jp/records/80212
https://repo.qst.go.jp/records/802120d2bbe6c-8e37-40e9-92fa-a85d4b198b75
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-07-16 | |||||
| タイトル | ||||||
| タイトル | X-ray Structural Study on Polyketide Synthase Involved in Olivetolic Acid Biosynthesis in Cannabis sativa | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kono, Fumiaki
× Kono, Fumiaki× Taguchi, Chiho× Blaber, Michael× Kuroki, Ryota× Morimoto, Satoshi× Tamada, Taro× Kono, Fumiaki× Tamada, Taro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Cannabis sativa L. produces unique secondary metabolites known as cannabinoids which have psychotropic and pharmacological activities. Major cannabinoids including DELTA9-tetrahydrocannabinol are biosynthesized from a common precursor olivetolic acid. A type III polyketide synthetase (PKS) from C. sativa named PKS-1 is considered to synthesize the tetraketide intermediate of olivetolic acid by condensation of hexanoyl-CoA with three malonyl-CoA molecules. Whereas most plant PKSs can react with various CoA esters, PKS-1 exhibits a relatively strict substrate specificity for hexanoyl-CoA as the starter substrate. The unique enzymatic property of PKS-1 is important in the cannabinoid pathway. To clarify the structure-function relationship of PKS-1, we have determined X-ray crystal structures of recombinant apo PKS-1 and complexes with hexanoyl-CoA or malonyl-CoA, at resolutions of 1.6 Å, 1.6 Å and 1.8 Å, respectively. The overall structure of PKS-1 is similar to those of other plant PKSs. As for the loop regions around the active site of PKS-1, which are thought to characterize the enzymatic properties, their structures are most similar to those of pyrone synthase from Gerbera hybrida. The binding pattern of the CoA moiety to PKS-1 is similar between hexanoyl-CoA and malonyl-CoA. Compared to other plant PKSs, several amino acid side chains of PKS-1 that constitute the active site cavity are positioned to effectively reduce the cavity volume and bind the aliphatic moiety of hexanoyl-CoA tightly. These structural features of PKS-1 are considered to enable selective binding of hexanoyl-CoA leading to production of cannabinoids unique to C. sativa. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第20回日本蛋白質科学会年会 | |||||
| 発表年月日 | ||||||
| 日付 | 2020-07-15 | |||||
| 日付タイプ | Issued | |||||
