{"created":"2023-05-15T14:59:07.205756+00:00","id":80212,"links":{},"metadata":{"_buckets":{"deposit":"f06d805c-fefa-4741-9c29-f4a5489ee093"},"_deposit":{"created_by":1,"id":"80212","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"80212"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00080212","sets":["10:28"]},"author_link":["878466","878469","878467","878471","878470","878473","878468","878472"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-07-15","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cannabis sativa L. produces unique secondary metabolites known as cannabinoids which have psychotropic and pharmacological activities. Major cannabinoids including DELTA9-tetrahydrocannabinol are biosynthesized from a common precursor olivetolic acid. A type III polyketide synthetase (PKS) from C. sativa named PKS-1 is considered to synthesize the tetraketide intermediate of olivetolic acid by condensation of hexanoyl-CoA with three malonyl-CoA molecules. Whereas most plant PKSs can react with various CoA esters, PKS-1 exhibits a relatively strict substrate specificity for hexanoyl-CoA as the starter substrate. The unique enzymatic property of PKS-1 is important in the cannabinoid pathway. To clarify the structure-function relationship of PKS-1, we have determined X-ray crystal structures of recombinant apo PKS-1 and complexes with hexanoyl-CoA or malonyl-CoA, at resolutions of 1.6 Å, 1.6 Å and 1.8 Å, respectively. The overall structure of PKS-1 is similar to those of other plant PKSs. As for the loop regions around the active site of PKS-1, which are thought to characterize the enzymatic properties, their structures are most similar to those of pyrone synthase from Gerbera hybrida. The binding pattern of the CoA moiety to PKS-1 is similar between hexanoyl-CoA and malonyl-CoA. Compared to other plant PKSs, several amino acid side chains of PKS-1 that constitute the active site cavity are positioned to effectively reduce the cavity volume and bind the aliphatic moiety of hexanoyl-CoA tightly. These structural features of PKS-1 are considered to enable selective binding of hexanoyl-CoA leading to production of cannabinoids unique to C. sativa.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第20回日本蛋白質科学会年会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kono, Fumiaki"}],"nameIdentifiers":[{"nameIdentifier":"878466","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Taguchi, Chiho"}],"nameIdentifiers":[{"nameIdentifier":"878467","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Blaber, Michael"}],"nameIdentifiers":[{"nameIdentifier":"878468","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuroki, Ryota"}],"nameIdentifiers":[{"nameIdentifier":"878469","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Morimoto, Satoshi"}],"nameIdentifiers":[{"nameIdentifier":"878470","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tamada, Taro"}],"nameIdentifiers":[{"nameIdentifier":"878471","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kono, Fumiaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"878472","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tamada, Taro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"878473","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"X-ray Structural Study on Polyketide Synthase Involved in Olivetolic Acid Biosynthesis in Cannabis sativa","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"X-ray Structural Study on Polyketide Synthase Involved in Olivetolic Acid Biosynthesis in Cannabis sativa"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-07-16"},"publish_date":"2020-07-16","publish_status":"0","recid":"80212","relation_version_is_last":true,"title":["X-ray Structural Study on Polyketide Synthase Involved in Olivetolic Acid Biosynthesis in Cannabis sativa"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:44:08.888433+00:00"}