WEKO3
アイテム
Participation of TDP1 in the repair of formaldehyde-induced DNA-protein cross- links in chicken DT40 cells
https://repo.qst.go.jp/records/80117
https://repo.qst.go.jp/records/801176c2458cb-6846-48e8-abb1-3bf1ae801c93
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-06-29 | |||||
| タイトル | ||||||
| タイトル | Participation of TDP1 in the repair of formaldehyde-induced DNA-protein cross- links in chicken DT40 cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakano, Toshiaki
× Nakano, Toshiaki× I. Shoulkamy, Mahmoud× Tsuda, Masataka× Sasanuma, Hiroyuki× Hirota, Kouji× Takata, Minoru× Shin-ichiro, Masunaga× Takeda, Shunichi× Ide, Hiroshi× Bessho, Tadayoshi× Tano, Keizo× Nakano, Toshiaki |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Proteins are covalently trapped on DNA to form DNA-protein cross-links (DPCs) when cells are exposed to DNA-damaging agents. Aldehyde compounds produce common types of DPCs that contain proteins in an undisrupted DNA strand. Tyrosyl-DNA phosphodiesterase 1 (TDP1) repairs topoisomerase 1 (TOPO1) that is trapped at the 3’-end of DNA. In the pres- ent study, we examined the contribution of TDP1 to the repair of formaldehyde-induced DPCs using a reverse genetic strategy with chicken DT40 cells. The results obtained showed that cells deficient in TDP1 were sensitive to formaldehyde. The removal of formal- dehyde-induced DPCs was slower in tdp1-deficient cells than in wild type cells. We also found that formaldehyde did not produce trapped TOPO1, indicating that trapped TOPO1 was not a primary cytotoxic DNA lesion that was generated by formaldehyde and repaired by TDP1. The formaldehyde treatment resulted in the accumulation of chromosomal break- ages that were more prominent in tdp1-deficient cells than in wild type cells. Therefore, TDP1 plays a critical role in the repair of formaldehyde-induced DPCs that are distinct from trapped TOPO1. | |||||
| 書誌情報 |
PLOS ONE 巻 15, 号 6, p. e0234859, 発行日 2020-06 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1932-6203 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1371/journal.pone.0234859 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0234859 | |||||
