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Participation of TDP1 in the repair of formaldehyde-induced DNA-protein cross- links in chicken DT40 cells
https://repo.qst.go.jp/records/80117
https://repo.qst.go.jp/records/801176c2458cb-6846-48e8-abb1-3bf1ae801c93
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-06-29 | |||||
タイトル | ||||||
タイトル | Participation of TDP1 in the repair of formaldehyde-induced DNA-protein cross- links in chicken DT40 cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Nakano, Toshiaki
× Nakano, Toshiaki× I. Shoulkamy, Mahmoud× Tsuda, Masataka× Sasanuma, Hiroyuki× Hirota, Kouji× Takata, Minoru× Shin-ichiro, Masunaga× Takeda, Shunichi× Ide, Hiroshi× Bessho, Tadayoshi× Tano, Keizo× Nakano, Toshiaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Proteins are covalently trapped on DNA to form DNA-protein cross-links (DPCs) when cells are exposed to DNA-damaging agents. Aldehyde compounds produce common types of DPCs that contain proteins in an undisrupted DNA strand. Tyrosyl-DNA phosphodiesterase 1 (TDP1) repairs topoisomerase 1 (TOPO1) that is trapped at the 3’-end of DNA. In the pres- ent study, we examined the contribution of TDP1 to the repair of formaldehyde-induced DPCs using a reverse genetic strategy with chicken DT40 cells. The results obtained showed that cells deficient in TDP1 were sensitive to formaldehyde. The removal of formal- dehyde-induced DPCs was slower in tdp1-deficient cells than in wild type cells. We also found that formaldehyde did not produce trapped TOPO1, indicating that trapped TOPO1 was not a primary cytotoxic DNA lesion that was generated by formaldehyde and repaired by TDP1. The formaldehyde treatment resulted in the accumulation of chromosomal break- ages that were more prominent in tdp1-deficient cells than in wild type cells. Therefore, TDP1 plays a critical role in the repair of formaldehyde-induced DPCs that are distinct from trapped TOPO1. | |||||
書誌情報 |
PLOS ONE 巻 15, 号 6, p. e0234859, 発行日 2020-06 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1932-6203 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pone.0234859 | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0234859 |