{"created":"2023-05-15T14:59:03.178060+00:00","id":80117,"links":{},"metadata":{"_buckets":{"deposit":"602d71c8-54fa-4657-871b-3ce2a4eb5bf5"},"_deposit":{"created_by":1,"id":"80117","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"80117"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00080117","sets":["1"]},"author_link":["878288","878286","878283","878284","878278","878287","878285","878279","878280","878282","878277","878281"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageStart":"e0234859","bibliographicVolumeNumber":"15","bibliographic_titles":[{"bibliographic_title":"PLOS ONE"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Proteins are covalently trapped on DNA to form DNA-protein cross-links (DPCs) when cells are exposed to DNA-damaging agents. Aldehyde compounds produce common types of DPCs that contain proteins in an undisrupted DNA strand. Tyrosyl-DNA phosphodiesterase 1 (TDP1) repairs topoisomerase 1 (TOPO1) that is trapped at the 3’-end of DNA. In the pres- ent study, we examined the contribution of TDP1 to the repair of formaldehyde-induced DPCs using a reverse genetic strategy with chicken DT40 cells. The results obtained showed that cells deficient in TDP1 were sensitive to formaldehyde. The removal of formal- dehyde-induced DPCs was slower in tdp1-deficient cells than in wild type cells. We also found that formaldehyde did not produce trapped TOPO1, indicating that trapped TOPO1 was not a primary cytotoxic DNA lesion that was generated by formaldehyde and repaired by TDP1. The formaldehyde treatment resulted in the accumulation of chromosomal break- ages that were more prominent in tdp1-deficient cells than in wild type cells. Therefore, TDP1 plays a critical role in the repair of formaldehyde-induced DPCs that are distinct from trapped TOPO1.\n","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0234859","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0234859","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakano, Toshiaki"}],"nameIdentifiers":[{"nameIdentifier":"878277","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"I. Shoulkamy, Mahmoud"}],"nameIdentifiers":[{"nameIdentifier":"878278","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuda, Masataka"}],"nameIdentifiers":[{"nameIdentifier":"878279","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasanuma, Hiroyuki"}],"nameIdentifiers":[{"nameIdentifier":"878280","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirota, Kouji"}],"nameIdentifiers":[{"nameIdentifier":"878281","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takata, Minoru"}],"nameIdentifiers":[{"nameIdentifier":"878282","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shin-ichiro, Masunaga"}],"nameIdentifiers":[{"nameIdentifier":"878283","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takeda, Shunichi"}],"nameIdentifiers":[{"nameIdentifier":"878284","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ide, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"878285","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Bessho, Tadayoshi"}],"nameIdentifiers":[{"nameIdentifier":"878286","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tano, Keizo"}],"nameIdentifiers":[{"nameIdentifier":"878287","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakano, Toshiaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"878288","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Participation of TDP1 in the repair of formaldehyde-induced DNA-protein cross- links in chicken DT40 cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Participation of TDP1 in the repair of formaldehyde-induced DNA-protein cross- links in chicken DT40 cells"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-06-29"},"publish_date":"2020-06-29","publish_status":"0","recid":"80117","relation_version_is_last":true,"title":["Participation of TDP1 in the repair of formaldehyde-induced DNA-protein cross- links in chicken DT40 cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:44:29.133224+00:00"}