WEKO3
アイテム
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FACS analysis showed that the proportion of cancer stem-like marker CD44+ and ESA+ cells were 4.8% and 0.9%, but CD133+ was undetectable. The proportion of CD44+, ESA+ cells was more significantly enriched by X-rays or gemcitabine treatment compared to carbon-ion irradiation. The proportion of CD44+ cells did not change after treatment with PI3K inhibitor LY29402 or MAPK inhibitor PD98059 only, but remarkably enriched in combination with X-rays or C-ion irradiation, suggesting that PI3K-AKt survival pathway as well as MAPK kinase pathway are involved in IR induced enrichment of CSC. CD44+/CD24+ and CD44+/ESA+ cells significantly have higher number of colony and spheroid compared to CD44-/CD24- and CD44-/ESA- cells. CD44+/CD24+ and CD44+/ESA+ cells showed radioresistance to both X-ray and carbon ion irradiation, but carbon ion irradiation was more effective at killing those of cancer stem-like cells. CD44+/CD24+ and CD44+/ESA+ cells have shown more potential to form tumors in SCID mice than CD44-/CD24- and CD44-/ESA- cells, and carbon ion beam more effectively eradicates xenograft tumors compared to X-rays at doses of same biological efficacy. \nConclusion:Carbon ion irradiation has potential advantages over X-rays in targeting radioresistant pancreatic cancer stem-like cells.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "The 4th Asian-Oceanic Pancreatic Association (AOPA)", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sai, Sei"}], "nameIdentifiers": [{"nameIdentifier": "695676", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oonishi, Kazuhiko"}], "nameIdentifiers": [{"nameIdentifier": "695677", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada, Shigeru"}], "nameIdentifiers": [{"nameIdentifier": "695678", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kamada, Tadashi"}], "nameIdentifiers": [{"nameIdentifier": "695679", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "崔 星", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "695680", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "大西 和彦", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "695681", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "山田 滋", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "695682", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鎌田 正", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "695683", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "Effects of carbon ion and X-ray irradiation on pancreatic cancer stem cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Effects of carbon ion and X-ray irradiation on pancreatic cancer stem cells"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/70809", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-06-22"}, "publish_date": "2012-06-22", "publish_status": "0", "recid": "70809", "relation": {}, "relation_version_is_last": true, "title": ["Effects of carbon ion and X-ray irradiation on pancreatic cancer stem cells"], "weko_shared_id": -1}
Effects of carbon ion and X-ray irradiation on pancreatic cancer stem cells
https://repo.qst.go.jp/records/70809
https://repo.qst.go.jp/records/70809895164bd-0c1c-4b91-993a-17f02a92bc13
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2012-06-22 | |||||
タイトル | ||||||
タイトル | Effects of carbon ion and X-ray irradiation on pancreatic cancer stem cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Sai, Sei
× Sai, Sei× Oonishi, Kazuhiko× Yamada, Shigeru× Kamada, Tadashi× 崔 星× 大西 和彦× 山田 滋× 鎌田 正 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Purpose:To investigate whether a carbon ion beam has beneficial effects compared to X-rays by targeting putative human pancreatic cancer stem cells. Materials:Human pancreatic cancer stem-like cells sorted from PANC1 and PK45 cells were treated with or without carbon ion or X-ray irradiation and then colony formation assay, spheroid formation assay, FACS analysis, as well as xenograft tumor growth control analyses were performed. Results:The in vitro relative biological effectiveness (RBE) values of carbon ion beams relative to X-rays calculated by the D10 levels were 1.71 to 2.18. FACS analysis showed that the proportion of cancer stem-like marker CD44+ and ESA+ cells were 4.8% and 0.9%, but CD133+ was undetectable. The proportion of CD44+, ESA+ cells was more significantly enriched by X-rays or gemcitabine treatment compared to carbon-ion irradiation. The proportion of CD44+ cells did not change after treatment with PI3K inhibitor LY29402 or MAPK inhibitor PD98059 only, but remarkably enriched in combination with X-rays or C-ion irradiation, suggesting that PI3K-AKt survival pathway as well as MAPK kinase pathway are involved in IR induced enrichment of CSC. CD44+/CD24+ and CD44+/ESA+ cells significantly have higher number of colony and spheroid compared to CD44-/CD24- and CD44-/ESA- cells. CD44+/CD24+ and CD44+/ESA+ cells showed radioresistance to both X-ray and carbon ion irradiation, but carbon ion irradiation was more effective at killing those of cancer stem-like cells. CD44+/CD24+ and CD44+/ESA+ cells have shown more potential to form tumors in SCID mice than CD44-/CD24- and CD44-/ESA- cells, and carbon ion beam more effectively eradicates xenograft tumors compared to X-rays at doses of same biological efficacy. Conclusion:Carbon ion irradiation has potential advantages over X-rays in targeting radioresistant pancreatic cancer stem-like cells. |
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会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | The 4th Asian-Oceanic Pancreatic Association (AOPA) | |||||
発表年月日 | ||||||
日付 | 2011-09-03 | |||||
日付タイプ | Issued |