{"created":"2023-05-15T14:51:47.917592+00:00","id":70809,"links":{},"metadata":{"_buckets":{"deposit":"c23e8a94-f690-4f53-a25b-effc6bc83f87"},"_deposit":{"created_by":1,"id":"70809","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70809"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070809","sets":["10:28"]},"author_link":["695676","695679","695677","695682","695678","695681","695680","695683"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-09-03","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose:To investigate whether a carbon ion beam has beneficial effects compared to X-rays by targeting putative human pancreatic cancer stem cells. \nMaterials:Human pancreatic cancer stem-like cells sorted from PANC1 and PK45 cells were treated with or without carbon ion or X-ray irradiation and then colony formation assay, spheroid formation assay, FACS analysis, as well as xenograft tumor growth control analyses were performed.  \nResults:The in vitro relative biological effectiveness (RBE) values of carbon ion beams relative to X-rays calculated by the D10 levels were 1.71 to 2.18. FACS analysis showed that the proportion of cancer stem-like marker CD44+ and ESA+ cells were 4.8% and 0.9%, but CD133+ was undetectable. The proportion of CD44+, ESA+ cells was more significantly enriched by X-rays or gemcitabine treatment compared to carbon-ion irradiation. The proportion of CD44+ cells did not change after treatment with PI3K inhibitor LY29402 or MAPK inhibitor PD98059 only, but remarkably enriched in combination with X-rays or C-ion irradiation, suggesting that PI3K-AKt survival pathway as well as MAPK kinase pathway are involved in IR induced enrichment of CSC. CD44+/CD24+ and CD44+/ESA+ cells significantly have higher number of colony and spheroid compared to CD44-/CD24- and CD44-/ESA- cells. CD44+/CD24+ and CD44+/ESA+ cells showed radioresistance to both X-ray and carbon ion irradiation, but carbon ion irradiation was more effective at killing those of cancer stem-like cells. CD44+/CD24+ and CD44+/ESA+ cells have shown more potential to form tumors in SCID mice than CD44-/CD24- and CD44-/ESA- cells, and carbon ion beam more effectively eradicates xenograft tumors compared to X-rays at doses of same biological efficacy. \nConclusion:Carbon ion irradiation has potential advantages over X-rays in targeting radioresistant pancreatic cancer stem-like cells.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"The 4th Asian-Oceanic Pancreatic Association (AOPA)","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"695676","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oonishi, Kazuhiko"}],"nameIdentifiers":[{"nameIdentifier":"695677","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Shigeru"}],"nameIdentifiers":[{"nameIdentifier":"695678","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamada, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"695679","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"695680","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大西 和彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"695681","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 滋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"695682","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田 正","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"695683","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Effects of carbon ion and X-ray irradiation on pancreatic cancer stem cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of carbon ion and X-ray irradiation on pancreatic cancer stem cells"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-06-22"},"publish_date":"2012-06-22","publish_status":"0","recid":"70809","relation_version_is_last":true,"title":["Effects of carbon ion and X-ray irradiation on pancreatic cancer stem cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:58:35.022333+00:00"}