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Tumor metastasis exposed to high-LET radiations
https://repo.qst.go.jp/records/64014
https://repo.qst.go.jp/records/640143eb1a02e-b15b-4d17-bb6e-7feaca646695
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-09-22 | |||||
| タイトル | ||||||
| タイトル | Tumor metastasis exposed to high-LET radiations | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Matsumoto, Yoshitaka
× Matsumoto, Yoshitaka× Uzawa, Akiko× Hirayama, Ryoichi× Koike, Sachiko× Okayasu, Ryuichi× Ando, Koichi× Furusawa, Yoshiya× et.al× 松本 孔貴× 鵜澤 玲子× 平山 亮一× 小池 幸子× 岡安 隆一× 古澤 佳也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: The aim of this study is to clarify the effect of carbon ion beams (C-ion) on metastatic potential of melanoma in vitro and in vivo. Materials and Methods: A highly metastatic mouse malignant melanoma cell line B16/BL6 were maintained in RPMI-1640 medium supplemented with 10% FBS and antibiotics. [in vitro] Samples were prepared 2 days before and then irradiated with C-ions or X-rays. Surviving fractions were obtained using colony formation assay. Migration and invasion activity as metastatic potentials of the cells were examined using the Boyden-chamber method and the Matrigel invasion assay. [in vivo] The cells were implanted in right-leg of C57BL/6J mice at 1x106 cells/mouse 9-10 days before irradiation. Tumors were irradiated at the center of 6cm-SOBP of C-ions, or gamma-rays. Radiosensitivity for whole tumor was obtained by the tumor growth delay method, and that for individual cell in a tumor was obtained by an in vivo-in vitro assay. The metastatic effects were analyzed with the spontaneous lung metastasis model. The dose averaged LET values of carbon beams were approximately 50 keV/um. Results: [in vitro] Survival curves showed higher cytotoxic effects of C-ions compared with X-rays, and the RBE values were 1.96. The potential of migration and invasion were suppressed by C-ions at all dose points (0.50 to 8.0 Gy) tested, however it was enhanced by X-rays at low dose points (0.50 and 1.0 Gy) than non-irradiated controls. The RBE values obtained from migration and invasion test were higher than that from cell killing. [in vivo] C-ions significantly suppressed the tumor growth, and the RBE was 2.64. The numbers of lung metastatic nodules after tumor-irradiations decreased with the dose, and C-ions were more effective compared with gamma-rays. The metastatic potentials of survived cells in a tumor after irradiation was analyzed with the number of metastatic lung colony from implanted tumors and survival of irradiated and explanted cells from a tumor. Smaller number of metastasis was found for C-ions than gamma-rays when the numbers were compared with biological equivalent dose. Conclusion: It might suggest that C-ion inhibit metastasis at radiotherapy compared with low-LET photons. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | NIRS-KI Joint Symposium on Carbon Ion Therapy | |||||
| 発表年月日 | ||||||
| 日付 | 2010-09-11 | |||||
| 日付タイプ | Issued | |||||
