{"created":"2023-05-15T14:46:39.399851+00:00","id":64014,"links":{},"metadata":{"_buckets":{"deposit":"45f53c0b-c545-41ee-9dbd-880ae8f531a8"},"_deposit":{"created_by":1,"id":"64014","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"64014"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00064014","sets":["10:29"]},"author_link":["631543","631541","631548","631549","631551","631542","631547","631552","631546","631540","631545","631550","631539","631544"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-09-11","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose:  The aim of this study is to clarify the effect of carbon ion beams (C-ion) on metastatic potential of melanoma in vitro and in vivo. \nMaterials and Methods:  A highly metastatic mouse malignant melanoma cell line B16/BL6 were maintained in RPMI-1640 medium supplemented with 10% FBS and antibiotics. [in vitro] Samples were prepared 2 days before and then irradiated with C-ions or X-rays. Surviving fractions were obtained using colony formation assay. Migration and invasion activity as metastatic potentials of the cells were examined using the Boyden-chamber method and the Matrigel invasion assay. [in vivo] The cells were implanted in right-leg of C57BL/6J mice at 1x106 cells/mouse 9-10 days before irradiation. Tumors were irradiated at the center of 6cm-SOBP of C-ions, or gamma-rays. Radiosensitivity for whole tumor was obtained by the tumor growth delay method, and that for individual cell in a tumor was obtained by an in vivo-in vitro assay. The metastatic effects were analyzed with the spontaneous lung metastasis model. The dose averaged LET values of carbon beams were approximately 50 keV/um.\nResults:  [in vitro] Survival curves showed higher cytotoxic effects of C-ions compared with X-rays, and the RBE values were 1.96. The potential of migration and invasion were suppressed by C-ions at all dose points (0.50 to 8.0 Gy) tested, however it was enhanced by X-rays at low dose points (0.50 and 1.0 Gy) than non-irradiated controls. The RBE values obtained from migration and invasion test were higher than that from cell killing.  [in vivo] C-ions significantly suppressed the tumor growth, and the RBE was 2.64.  The numbers of lung metastatic nodules after tumor-irradiations decreased with the dose, and C-ions were more effective compared with gamma-rays.  The metastatic potentials of survived cells in a tumor after irradiation was analyzed with the number of metastatic lung colony from implanted tumors and survival of irradiated and explanted cells from a tumor. Smaller number of metastasis was found for C-ions than gamma-rays when the numbers were compared with biological equivalent dose.  \nConclusion:  It might suggest that C-ion inhibit metastasis at radiotherapy compared with low-LET photons.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"NIRS-KI  Joint Symposium on Carbon Ion Therapy","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matsumoto, Yoshitaka"}],"nameIdentifiers":[{"nameIdentifier":"631539","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Uzawa, Akiko"}],"nameIdentifiers":[{"nameIdentifier":"631540","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirayama, Ryoichi"}],"nameIdentifiers":[{"nameIdentifier":"631541","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koike, Sachiko"}],"nameIdentifiers":[{"nameIdentifier":"631542","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"631543","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ando, Koichi"}],"nameIdentifiers":[{"nameIdentifier":"631544","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furusawa, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"631545","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"631546","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 孔貴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"631547","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鵜澤 玲子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"631548","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平山 亮一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"631549","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小池 幸子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"631550","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"631551","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"631552","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Tumor metastasis exposed to high-LET radiations","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Tumor metastasis exposed to high-LET radiations"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-09-22"},"publish_date":"2010-09-22","publish_status":"0","recid":"64014","relation_version_is_last":true,"title":["Tumor metastasis exposed to high-LET radiations"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:16:24.328645+00:00"}