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gpt delta transgenic mice for in vivo genotoxicity assays: application for analysis of combined effects of radiation and chemicals
https://repo.qst.go.jp/records/63580
https://repo.qst.go.jp/records/6358054d3313e-ea4d-446a-8fef-cf2d20be4b4e
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-10-02 | |||||
| タイトル | ||||||
| タイトル | gpt delta transgenic mice for in vivo genotoxicity assays: application for analysis of combined effects of radiation and chemicals | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ikeda, Megumi
× Ikeda, Megumi× Masumura, Kenichi× Sakamoto, Yasuteru× Bing, Wang× Nenoi, Mitsuru× Hayata, Isamu× Honma, Masamitsu× Nohmi, Takehiko× et.al× 池田 恵× 増村 健一× 坂元 康晃× 王 冰× 根井 充× 早田 勇× 能美 健彦 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | It is important to evaluate the combined effects of low–dose-rate or low-dose radiation with chemicals as humans are exposed to a variety of chemical agents. Here, we applied gpt delta transgenic genotoxicity assay to examine combined genotoxic effects of low-dose-rate radiation and 4-(methylnitrosamino)-1-(3-pyridy1)-1-butanone (NNK), the most carcinogenic tobacco-specific nitrosamine and a methylating agent, in the lung of mice. In this mouse model, base substitutions and deletions can be separately analyzed by gpt and Spi- selections, respectively. Female gpt delta mice were treated either with gamma-irradiation alone at a dose rate of 0.5, 1.0 or 1.5mGy/h for 22 h/day for 31 days or its combination with NNK at a dose of 2mg/mouse/day, i.p. for four consecutive days in the middle course of irradiation. In the gpt selection, the NNK treatments enhanced the mutation frequencies (MFs) significantly, but no obvious combined effects of gamma-irradiation were observable at any given radiation dose. In contrast, NNK treatments appeared to suppress the Spi- large deletions. In the Spi- selection, when NNK treatments were combined, the dose-response curve became bell-shaped where the highest radiation dose decreased substantially. These results suggest that NNK treatments may elicit an adaptive response that eliminates cells bearing radiation-induced double-strand breaks (DSBs) in DNA. We are currently examining the mechanisms underlying the apparent suppressive effects of methylating agent against radiation-induced DSBs with human cells in vitro. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 7th Japan-France Workshop on Radiation Biology | |||||
| 発表年月日 | ||||||
| 日付 | 2008-10-16 | |||||
| 日付タイプ | Issued | |||||
