{"created":"2023-05-15T14:46:18.961152+00:00","id":63580,"links":{},"metadata":{"_buckets":{"deposit":"640d330c-d387-42af-8e53-2ceb9fc70cdc"},"_deposit":{"created_by":1,"id":"63580","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"63580"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00063580","sets":["10:29"]},"author_link":["627501","627507","627508","627513","627504","627515","627512","627509","627510","627503","627514","627506","627505","627511","627516","627502"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-10-16","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"It is important to evaluate the combined effects of low–dose-rate or low-dose radiation with chemicals as humans are exposed to a variety of chemical agents. Here, we applied gpt delta transgenic genotoxicity assay to examine combined genotoxic effects of low-dose-rate radiation and 4-(methylnitrosamino)-1-(3-pyridy1)-1-butanone (NNK), the most carcinogenic tobacco-specific nitrosamine and a methylating agent, in the lung of mice. In this mouse model, base substitutions and deletions can be separately analyzed by gpt and Spi- selections, respectively. Female gpt delta mice were treated either with gamma-irradiation alone at a dose rate of 0.5, 1.0 or 1.5mGy/h for 22 h/day for 31 days or its combination with NNK at a dose of 2mg/mouse/day, i.p. for four consecutive days in the middle course of irradiation. In the gpt selection, the NNK treatments enhanced the mutation frequencies (MFs) significantly, but no obvious combined effects of gamma-irradiation were observable at any given radiation dose. In contrast, NNK treatments appeared to suppress the Spi- large deletions. In the Spi- selection, when NNK treatments were combined, the dose-response curve became bell-shaped where the highest radiation dose decreased substantially. These results suggest that NNK treatments may elicit an adaptive response that eliminates cells bearing radiation-induced double-strand breaks (DSBs) in DNA. We are currently examining the mechanisms underlying the apparent suppressive effects of methylating agent against radiation-induced DSBs with human cells in vitro.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"The 7th Japan-France Workshop on Radiation Biology","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ikeda, Megumi"}],"nameIdentifiers":[{"nameIdentifier":"627501","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Masumura, Kenichi"}],"nameIdentifiers":[{"nameIdentifier":"627502","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakamoto, Yasuteru"}],"nameIdentifiers":[{"nameIdentifier":"627503","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Bing, Wang"}],"nameIdentifiers":[{"nameIdentifier":"627504","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nenoi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"627505","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hayata, Isamu"}],"nameIdentifiers":[{"nameIdentifier":"627506","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Honma, Masamitsu"}],"nameIdentifiers":[{"nameIdentifier":"627507","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nohmi, Takehiko"}],"nameIdentifiers":[{"nameIdentifier":"627508","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"627509","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"池田 恵","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627510","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"増村 健一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627511","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"坂元 康晃","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627512","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"王 冰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627513","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627514","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"早田 勇","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627515","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"能美 健彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"627516","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"gpt delta transgenic mice for in vivo genotoxicity assays: application for analysis of combined effects of radiation and chemicals","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"gpt delta transgenic mice for in vivo genotoxicity assays: application for analysis of combined effects of radiation and chemicals"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-10-02"},"publish_date":"2009-10-02","publish_status":"0","recid":"63580","relation_version_is_last":true,"title":["gpt delta transgenic mice for in vivo genotoxicity assays: application for analysis of combined effects of radiation and chemicals"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:21:21.341293+00:00"}