WEKO3
アイテム
Regulation of the Catalase Gene Promoter by Sp1, CCAAT-Recognizing Factors, and a WT1/Egr-Related Factor in Hydrogen Peroxide-Resistant HP100 Cells.
https://repo.qst.go.jp/records/60132
https://repo.qst.go.jp/records/601323a2761c0-0dfa-4e9b-b85e-293906326db3
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-11-14 | |||||
| タイトル | ||||||
| タイトル | Regulation of the Catalase Gene Promoter by Sp1, CCAAT-Recognizing Factors, and a WT1/Egr-Related Factor in Hydrogen Peroxide-Resistant HP100 Cells. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nenoi, Mitsuru
× Nenoi, Mitsuru× Ichimura, Sachiko× Mita, Kazuei× Yukawa, Osami× L., Cartwright Iain× 根井 充× 沼田 幸子× 三田 和英× 湯川 修身 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Reactive oxygen species (ROS) play a critical role in the onset of apoptosis induced by various extracellular stimuli, including ionizing radiation. Therefore active regulation of ROS-metabolizing enzymes may be one response to an apoptotic stimulus. In this regard HP100 cells, H2O2-resistant variants derived from human leukemia HL60 cells, display an interesting phenotype wherein the activity of catalase is constitutively high, while its mRNA is reduced after X ray-irradiation. In the present study, we investigated the molecular mechanisms underlying this phenomenon. By combining analyses from nuclear run-on, reporter gene transient transfection, genomic footprinting, site-directed mutagenesis, EMSA, and Western blotting experiments, we found that constitutively elevated catalase expression is strongly regulated at the transcriptional level by both Sp1 and CCAAT-recognizing factors, and that much higher levels of nuclear Sp1 and NF-Y are present in HP100 as compared to HL60 nuclei. In addition, we demonstrated an X ray-inducible association of a WT1/Egr-related factor with an overlapping Sp1/Egr-1 recognition sequence located within the core promoter of the catalase gene. This association may lead to inactivation of the promoter by disturbing, or competing with, the transactivating ability of Sp1. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 41st Annual Meeting, The American Society for Cell Biology | |||||
| 発表年月日 | ||||||
| 日付 | 2001-12-12 | |||||
| 日付タイプ | Issued | |||||
