WEKO3
アイテム
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Regulation of the Catalase Gene Promoter by Sp1, CCAAT-Recognizing Factors, and a WT1/Egr-Related Factor in Hydrogen Peroxide-Resistant HP100 Cells.
https://repo.qst.go.jp/records/60132
https://repo.qst.go.jp/records/601323a2761c0-0dfa-4e9b-b85e-293906326db3
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2003-11-14 | |||||
タイトル | ||||||
タイトル | Regulation of the Catalase Gene Promoter by Sp1, CCAAT-Recognizing Factors, and a WT1/Egr-Related Factor in Hydrogen Peroxide-Resistant HP100 Cells. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Nenoi, Mitsuru
× Nenoi, Mitsuru× Ichimura, Sachiko× Mita, Kazuei× Yukawa, Osami× L., Cartwright Iain× 根井 充× 沼田 幸子× 三田 和英× 湯川 修身 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Reactive oxygen species (ROS) play a critical role in the onset of apoptosis induced by various extracellular stimuli, including ionizing radiation. Therefore active regulation of ROS-metabolizing enzymes may be one response to an apoptotic stimulus. In this regard HP100 cells, H2O2-resistant variants derived from human leukemia HL60 cells, display an interesting phenotype wherein the activity of catalase is constitutively high, while its mRNA is reduced after X ray-irradiation. In the present study, we investigated the molecular mechanisms underlying this phenomenon. By combining analyses from nuclear run-on, reporter gene transient transfection, genomic footprinting, site-directed mutagenesis, EMSA, and Western blotting experiments, we found that constitutively elevated catalase expression is strongly regulated at the transcriptional level by both Sp1 and CCAAT-recognizing factors, and that much higher levels of nuclear Sp1 and NF-Y are present in HP100 as compared to HL60 nuclei. In addition, we demonstrated an X ray-inducible association of a WT1/Egr-related factor with an overlapping Sp1/Egr-1 recognition sequence located within the core promoter of the catalase gene. This association may lead to inactivation of the promoter by disturbing, or competing with, the transactivating ability of Sp1. | |||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 41st Annual Meeting, The American Society for Cell Biology | |||||
発表年月日 | ||||||
日付 | 2001-12-12 | |||||
日付タイプ | Issued |