{"created":"2023-05-15T14:43:58.223091+00:00","id":60132,"links":{},"metadata":{"_buckets":{"deposit":"5bbe39ed-ca6f-47c9-9e48-756495f55d18"},"_deposit":{"created_by":1,"id":"60132","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"60132"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00060132","sets":["10:29"]},"author_link":["597908","597910","597913","597907","597912","597909","597914","597915","597911"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2001-12-12","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Reactive oxygen species (ROS) play a critical role in the onset of apoptosis induced by various extracellular stimuli, including ionizing radiation. Therefore active regulation of ROS-metabolizing enzymes may be one response to an apoptotic stimulus. In this regard HP100 cells, H2O2-resistant variants derived from human leukemia HL60 cells, display an interesting phenotype wherein the activity of catalase is constitutively high, while its mRNA is reduced after X ray-irradiation. In the present study, we investigated the molecular mechanisms underlying this phenomenon. By combining analyses from nuclear run-on, reporter gene transient transfection, genomic footprinting, site-directed mutagenesis, EMSA, and Western blotting experiments, we found that constitutively elevated catalase expression is strongly regulated at the transcriptional level by both Sp1 and CCAAT-recognizing factors, and that much higher levels of nuclear Sp1 and NF-Y are present in HP100 as compared to HL60 nuclei. In addition, we demonstrated an X ray-inducible association of a WT1/Egr-related factor with an overlapping Sp1/Egr-1 recognition sequence located within the core promoter of the catalase gene. This association may lead to inactivation of the promoter by disturbing, or competing with, the transactivating ability of Sp1.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"41st Annual Meeting, The American Society for Cell Biology","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nenoi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"597907","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ichimura, Sachiko"}],"nameIdentifiers":[{"nameIdentifier":"597908","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mita, Kazuei"}],"nameIdentifiers":[{"nameIdentifier":"597909","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yukawa, Osami"}],"nameIdentifiers":[{"nameIdentifier":"597910","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"L., Cartwright Iain"}],"nameIdentifiers":[{"nameIdentifier":"597911","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"597912","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"沼田 幸子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"597913","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"三田 和英","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"597914","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"湯川 修身","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"597915","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Regulation of the Catalase Gene Promoter by Sp1, CCAAT-Recognizing Factors, and a WT1/Egr-Related Factor in Hydrogen Peroxide-Resistant HP100 Cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Regulation of the Catalase Gene Promoter by Sp1, CCAAT-Recognizing Factors, and a WT1/Egr-Related Factor in Hydrogen Peroxide-Resistant HP100 Cells."}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-11-14"},"publish_date":"2003-11-14","publish_status":"0","recid":"60132","relation_version_is_last":true,"title":["Regulation of the Catalase Gene Promoter by Sp1, CCAAT-Recognizing Factors, and a WT1/Egr-Related Factor in Hydrogen Peroxide-Resistant HP100 Cells."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:57:09.243039+00:00"}