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Introduction of GFP-construct into medakafish using the particle gun method
https://repo.qst.go.jp/records/59871
https://repo.qst.go.jp/records/5987129d06dac-b087-41e2-a2e8-f1b18ed23cbb
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-07-14 | |||||
| タイトル | ||||||
| タイトル | Introduction of GFP-construct into medakafish using the particle gun method | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yamauchi, Masatake
× Yamauchi, Masatake× Sasanuma, Motoe× Tsuji, Satsuki× Terada, Minako× Ishikawa, Yuuji× 山内 正剛× 辻 さつき× 寺田 美奈子× 石川 裕二 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Medakafish (Oryzias latipes) belongs to a group of small oviparous teleost fishes that inhabit fresh water in Asian countries including Japan. Experimental procedures to introduce foreign genes into medakafish have been reported using electroporation and/or microinjection. Microinjection is widely used in gene transfer experiments. However, it requires high technical sophistication. Electroporation can be easily applied to gene transfer experiments, but with a low efficiency. Therefore, we attempted to develop an experimental procedure to introduce a foreign gene without high technical sophistication but at reasonably high efficiency. A particle gun is an instrument used to introduce DNA that is coated on gold microcarrier particles directly into cells at high speed using high-pressure gas. Here we report a procedure to introduce a foreign gene into fertilized eggs of medakafish using the particle gun method. A plasmid construct with the green fluorescence protein (GFP) gene driven by the medakafish beta-actin gene promoter was successfully introduced into eggs, and the expression of GFP was observed in 20% of the primary transfectant fish. In addition, germ line transmission of GFP was observed in 13% of the GFP-positive primary transfectant. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 12th International Sympojium on Bioluminescence and Chemiluminescence | |||||
| 発表年月日 | ||||||
| 日付 | 2002-04-09 | |||||
| 日付タイプ | Issued | |||||
