{"created":"2023-05-15T14:43:45.363055+00:00","id":59871,"links":{},"metadata":{"_buckets":{"deposit":"5b06f5ec-dbea-4961-a1f7-b20f153843b3"},"_deposit":{"created_by":1,"id":"59871","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"59871"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00059871","sets":["10:29"]},"author_link":["595697","595695","595696","595694","595691","595698","595693","595692","595699"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2002-04-09","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Medakafish (Oryzias latipes) belongs to a group of small oviparous teleost fishes that inhabit fresh water in Asian countries including Japan. Experimental procedures to introduce foreign genes into medakafish have been reported using electroporation and/or microinjection. Microinjection is widely used in gene transfer experiments. However, it requires high technical sophistication. Electroporation can be easily applied to gene transfer experiments, but with a low efficiency. Therefore, we attempted to develop an experimental procedure to introduce a foreign gene without high technical sophistication but at reasonably high efficiency. A particle gun is an instrument used to introduce DNA that is coated on gold microcarrier particles directly into cells at high speed using high-pressure gas. Here we report a procedure to introduce a foreign gene into fertilized eggs of medakafish using the particle gun method. A plasmid construct with the green fluorescence protein (GFP) gene driven by the medakafish beta-actin gene promoter was successfully introduced into eggs, and the expression of GFP was observed in 20% of the primary transfectant fish. In addition, germ line transmission of GFP was observed in 13% of the GFP-positive primary transfectant. ","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"12th International Sympojium on Bioluminescence and Chemiluminescence ","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamauchi, Masatake"}],"nameIdentifiers":[{"nameIdentifier":"595691","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasanuma, Motoe"}],"nameIdentifiers":[{"nameIdentifier":"595692","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuji, Satsuki"}],"nameIdentifiers":[{"nameIdentifier":"595693","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Terada, Minako"}],"nameIdentifiers":[{"nameIdentifier":"595694","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikawa, Yuuji"}],"nameIdentifiers":[{"nameIdentifier":"595695","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山内 正剛","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"595696","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 さつき","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"595697","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"寺田 美奈子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"595698","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川 裕二","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"595699","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Introduction of GFP-construct into medakafish using the particle gun method","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Introduction of GFP-construct into medakafish using the particle gun method"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-07-14"},"publish_date":"2003-07-14","publish_status":"0","recid":"59871","relation_version_is_last":true,"title":["Introduction of GFP-construct into medakafish using the particle gun method"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T22:00:05.572892+00:00"}