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Visualization of in vivo electroporation-mediated transgene expression in experimental tumors by optical and magnetic resonance imaging
https://repo.qst.go.jp/records/49237
https://repo.qst.go.jp/records/492376d09250c-459d-4309-a665-343f0308dae0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-11-02 | |||||
| タイトル | ||||||
| タイトル | Visualization of in vivo electroporation-mediated transgene expression in experimental tumors by optical and magnetic resonance imaging | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
U, Winn Aung
× U, Winn Aung× Hasegawa, Sumitaka× Koshikawa, Michiko× Obata, Takayuki× Ikehira, Hiroo× Furukawa, Takako× Aoki, Ichio× Saga, Tsuneo× Ｕ Ｗｉｎｎ Ａｕｎｇ× 長谷川 純崇× 越川 道子× 小畠 隆行× 池平 博夫× 古川 高子× 青木 伊知男× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In vivo electroporation (EP) is an efficient method for effective gene transfer and is highly expected for application in anticancer gene therapy. Non-invasive monitoring of gene transfer/expression is critical for optimal gene therapy. Here we report in vivo optical and high-field magnetic resonance imaging (MRI) of EP-mediated transgene expression in a tumor model. Initially, we observed spatio-temporal change in in vivo EP-mediated transgene expression by optical imaging using red fluorescence protein (RFP) as a reporter gene. Next, we constructed a dual-reporter plasmid carrying a gene-encoding MRI reporter ferritin heavy chain and RFP gene to visualize the intratumoral transgene expression by dual modality. Cells transfected with this plasmid showed lower signal intensity on in vitro T2-weighted cellular MRI and quantitatively increased the transverse relaxation rate (1/T2) compared with control cells. After conducting in vivo EP in an experimental tumor, the plasmidinjected region showed both fluorescent emissions in opticalimaging and detectably lowered signal on T2-weighted MRI. The correlative immunohistological findings confirmed that both the reporter transgenes were co-expressed in this region. Thus, our strategy provides a platform for evaluating EP-mediated cancer gene therapy easily and safely without administering contrast agent or substrate. |
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| 書誌情報 |
Gene Therapy 巻 16, 号 7, p. 830-839, 発行日 2009-05 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0969-7128 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 19458649 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1038/gt.2009.55 | |||||
