@article{oai:repo.qst.go.jp:00049237,
 author = {U, Winn Aung and Hasegawa, Sumitaka and Koshikawa, Michiko and Obata, Takayuki and Ikehira, Hiroo and Furukawa, Takako and Aoki, Ichio and Saga, Tsuneo and Ｕ Ｗｉｎｎ Ａｕｎｇ and 長谷川 純崇 and 越川 道子 and 小畠 隆行 and 池平 博夫 and 古川 高子 and 青木 伊知男 and 佐賀 恒夫},
 issue = {7},
 journal = {Gene Therapy},
 month = {May},
 note = {In vivo electroporation (EP) is an efficient method for effective gene transfer and is highly expected for application in anticancer gene therapy. Non-invasive monitoring of gene transfer/expression is critical for optimal gene therapy.
Here we report in vivo optical and high-field magnetic resonance imaging (MRI) of EP-mediated transgene expression in a tumor model. Initially, we observed spatio-temporal change in in vivo EP-mediated transgene expression by optical imaging using red fluorescence protein (RFP) as a reporter gene. Next, we constructed a dual-reporter plasmid carrying a gene-encoding MRI reporter ferritin heavy chain and RFP gene to visualize the intratumoral transgene expression by dual modality. Cells transfected with this plasmid showed lower signal intensity on in vitro T2-weighted cellular MRI and quantitatively increased the transverse relaxation rate (1/T2) compared with control cells. After conducting in vivo EP in an experimental tumor, the plasmidinjected region showed both fluorescent emissions in opticalimaging and detectably lowered signal on T2-weighted MRI.
The correlative immunohistological findings confirmed that both the reporter transgenes were co-expressed in this region. Thus, our strategy provides a platform for evaluating EP-mediated cancer gene therapy easily and safely without
administering contrast agent or substrate.},
 pages = {830--839},
 title = {Visualization of in vivo electroporation-mediated transgene expression in experimental tumors by optical and magnetic resonance imaging},
 volume = {16},
 year = {2009}
}