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In order to clarify the utility of [11C]acetate for atherosclerosis imaging, we determined the uptake and metabolism of acetate by in vitro studies using rabbit atherosclerotic arteries and [14C]acetate.\nMethods\nRabbits were fed with a conventional (n = 5) or a 0.5% cholesterol diet (n = 6). One side of the iliac–femoral arteries was injured by a balloon catheter. Radioactivity levels in the iliac–femoral arteries were measured after incubation in DMEM containing [1-14C]acetate for 60 min (% dpm/mg tissue). Radioactive components in the homogenized arteries were partitioned into aqueous, organic, and residue fractions by the Folch method, and analyzed by thin-layer chromatography (TLC).\nResults\nThe radioactivity level in the injured arteries of rabbits fed with the 0.5% cholesterol diet (atherosclerotic arteries) was significantly higher than that in either the non-injured or injured arteries of rabbits fed with the conventional diet (p \u003c 0.05) (% dpm/mg tissue: conventional diet groups; 0.022 ± 0.005 and 0.024 ± 0.007, cholesterol diet groups; 0.029 ± 0.007 and 0.034 ± 0.005 for non-injured and injured arteries). In metabolite analysis, most of the radioactivity was found in the aqueous fraction in each group (87.4–94.6% of total radioactivity in the arteries), and glutamate was a dominant component (67.4–69.7% of the aqueous fraction in the arteries).\nConclusions\nThe level of [14C]acetate-derived radioactivity into the arteries was increased by balloon injury and the burden of a cholesterol diet. Water-soluble metabolites were the dominant components with radioactivity in the atherosclerotic lesions. These results provide a biological basis for imaging atherosclerotic lesions by PET using [11C]acetate.", "subitem_description_type": "Abstract"}]}, "item_8_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "ELSEVIER"}]}, "item_8_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "29055850", "subitem_relation_type_select": "PMID"}}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.nucmedbio.2017.08.003", "subitem_relation_type_select": "DOI"}}]}, "item_8_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "https://doi.org/10.1016/j.nucmedbio.2017.08.003"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.nucmedbio.2017.08.003", "subitem_relation_type_select": "DOI"}}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yamasaki, Kazuaki"}], "nameIdentifiers": [{"nameIdentifier": "494436", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamashita, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "494437", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Zhao, Yan"}], "nameIdentifiers": [{"nameIdentifier": "494438", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimizu, Yoichi"}], "nameIdentifiers": [{"nameIdentifier": "494439", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishii, Ryuichi"}], "nameIdentifiers": [{"nameIdentifier": "494440", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawai, Keiichi"}], "nameIdentifiers": [{"nameIdentifier": "494441", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tamaki, Nagara"}], "nameIdentifiers": [{"nameIdentifier": "494442", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Zhao, Songji"}], "nameIdentifiers": [{"nameIdentifier": "494443", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Asada, Yujiro"}], "nameIdentifiers": [{"nameIdentifier": "494444", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuge, Yuji"}], "nameIdentifiers": [{"nameIdentifier": "494445", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "西井 龍一", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "494446", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "In vitro uptake and metabolism of [14C]acetate in rabbit atherosclerotic arteries: biological basis for atherosclerosis imaging with [11C]acetate", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "In vitro uptake and metabolism of [14C]acetate in rabbit atherosclerotic arteries: biological basis for atherosclerosis imaging with [11C]acetate"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/49026", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-05-17"}, "publish_date": "2018-05-17", "publish_status": "0", "recid": "49026", "relation": {}, "relation_version_is_last": true, "title": ["In vitro uptake and metabolism of [14C]acetate in rabbit atherosclerotic arteries: biological basis for atherosclerosis imaging with [11C]acetate"], "weko_shared_id": -1}
In vitro uptake and metabolism of [14C]acetate in rabbit atherosclerotic arteries: biological basis for atherosclerosis imaging with [11C]acetate
https://repo.qst.go.jp/records/49026
https://repo.qst.go.jp/records/490269f35d892-23af-45f4-a14a-f14917e855af
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-05-17 | |||||
タイトル | ||||||
タイトル | In vitro uptake and metabolism of [14C]acetate in rabbit atherosclerotic arteries: biological basis for atherosclerosis imaging with [11C]acetate | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Yamasaki, Kazuaki
× Yamasaki, Kazuaki× Yamashita, Atsushi× Zhao, Yan× Shimizu, Yoichi× Nishii, Ryuichi× Kawai, Keiichi× Tamaki, Nagara× Zhao, Songji× Asada, Yujiro× Kuge, Yuji× 西井 龍一 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Abstract Introduction Detection of vulnerable plaques is critically important for the selection of appropriate treatment and/or the prevention of atherosclerosis and ensuing cardiovascular diseases. In order to clarify the utility of [11C]acetate for atherosclerosis imaging, we determined the uptake and metabolism of acetate by in vitro studies using rabbit atherosclerotic arteries and [14C]acetate. Methods Rabbits were fed with a conventional (n = 5) or a 0.5% cholesterol diet (n = 6). One side of the iliac–femoral arteries was injured by a balloon catheter. Radioactivity levels in the iliac–femoral arteries were measured after incubation in DMEM containing [1-14C]acetate for 60 min (% dpm/mg tissue). Radioactive components in the homogenized arteries were partitioned into aqueous, organic, and residue fractions by the Folch method, and analyzed by thin-layer chromatography (TLC). Results The radioactivity level in the injured arteries of rabbits fed with the 0.5% cholesterol diet (atherosclerotic arteries) was significantly higher than that in either the non-injured or injured arteries of rabbits fed with the conventional diet (p < 0.05) (% dpm/mg tissue: conventional diet groups; 0.022 ± 0.005 and 0.024 ± 0.007, cholesterol diet groups; 0.029 ± 0.007 and 0.034 ± 0.005 for non-injured and injured arteries). In metabolite analysis, most of the radioactivity was found in the aqueous fraction in each group (87.4–94.6% of total radioactivity in the arteries), and glutamate was a dominant component (67.4–69.7% of the aqueous fraction in the arteries). Conclusions The level of [14C]acetate-derived radioactivity into the arteries was increased by balloon injury and the burden of a cholesterol diet. Water-soluble metabolites were the dominant components with radioactivity in the atherosclerotic lesions. These results provide a biological basis for imaging atherosclerotic lesions by PET using [11C]acetate. |
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書誌情報 |
Nuclear Medicine and Biology 巻 56, p. 21-25, 発行日 2018-01 |
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出版者 | ||||||
出版者 | ELSEVIER | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 29055850 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.nucmedbio.2017.08.003 | |||||
関連サイト | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.nucmedbio.2017.08.003 | |||||
関連名称 | https://doi.org/10.1016/j.nucmedbio.2017.08.003 |